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【求助】求一篇文献-有关HBV分子动力学模拟 已有1人参与
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Article Computational model of hepatitis B virus DNA polymerase: Molecular dynamics and docking to understand resistant mutations Protein Science Volume 19 Issue 4, Pages 796 - 807 Published Online: 16 Feb 2010 http://www3.interscience.wiley.com/journal/123283439/abstract |
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我就是申请一个面上项目而已，这评审意见是按照杰青的条件评的吧？
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有多少人是今天查系统知道结果的？
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售SCI文章，我:8O5.5.1.O.54,科目齐全,可+急
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售SCI一区T0P文章，我:8O.55.1.O.54,科目全,可伽急
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售SCI文章，我:8O5.5.1.O.54,科目齐全,可+急
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售SCI一区T0P文章，我:8O.55.1.O.54,科目全,可伽急
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售SCI-T0P文章，我:8O.5.5.1.O.54,科目齐全,可+急
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售SCI一区T0P文章，我:8.O.55.1.O.54,科目齐全,可+急
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售SCI文章，我:8O.5.5.1O.54,科目全,可十急
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ope123456789
铜虫 (小有名气)
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2楼2010-07-24 22:08:17
ope123456789
铜虫 (小有名气)
- 应助: 0 (幼儿园)
- 金币: 519
- 散金: 93
- 帖子: 232
- 在线: 31小时
- 虫号: 352130
- 注册: 2007-04-22
- 性别: GG
- 专业: 理论和计算化学
★ ★ ★
雪狼乖乖(金币+3):谢谢 2010-07-24 23:47:33
雪狼乖乖(金币+3):谢谢 2010-07-24 23:47:33
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Abstract Hepatitis B virus (HBV) DNA polymerase (HDP) is a pharmacological target of intense interest. Of the seven agents approved in USA for the treatment of HBV infections, five are HDP inhibitors. However, resistance development against HDP inhibitors, such as lamivudine and adefovir, has severely hurt their efficacy to treat HBV. As a step toward understanding the mechanism of resistance development and for gaining detailed insights about the active site of the enzyme, we have built a homology model of HDP which is an advance over previously reported ones. Validation using various techniques, including PROSTAT, PROCHECK, and Verify-3D profile, proved the model to be stereochemically significant. The stability of the model was studied using a 5 ns molecular dynamics simulation. The model was found to be sufficiently stable after the initial 2.5 ns with overall root mean squared deviation (RMSD) of 4.13 Å. The homology model matched the results of experimental mutation studies of HDP reported in the literature, including those of antiviral-resistant mutations. Our model suggests the significant role of conserved residues, such as rtLys32, in binding of the inhibitors, contrary to previous studies. The model provides an explanation for the inactivity of some anti-HIV molecules which are inactive against HDP. Conformational changes which occurred in certain binding pocket amino acids helped to explain the better binding of some of the inhibitors in comparison to the substrates. 不好意思只有摘要 |
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