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Response surface methodology, based on Box¨CBehnken design, was used to optimize the ultrasonic extraction conditions of Flammulina velutipes polysaccharides (FVP). Four independent variables (ratio of water to raw material, ultrasonic power, ultrasonic time, and ultrasonic temperature) were considered. Statistical analysis of the experiment indicated that the quadratic model fitted for the extraction conditions of FVP was in good agreement with the experimental results. A yield of FVP of 39.215% was obtain under the modified conditions (ratio of water to material 24 mL/g, ultrasonic power of 410 W, ultrasonic time of 25 min, and ultrasonic temperature of 35¡ãC). DPPH scavenging rate of 82.12% and AChE inhibitory rate of 18.51% obtained at 0.02g/mL in DPPH scavenging assay and AChE inhibitory activity test indicated a good potential of FVP to enhance cognitive ability.

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ÒÁÈËЦ(½ð±Ò+5):¶àл£¬ÎÒ»¹ÊÇһЩϸ½ÚûÓÐ×¢Òâµ½£¬ 2010-07-09 16:11:48
дµÃͦºÃµÄ£¬Ö»ÊÇ×öÁËÒ»µã¶ùСÐ޸ģ¬ÂÛÎÄÒ»°ã¶àÓÃÒ»°ãÏÖÔÚʱÀ´ËµÃ÷ÏÖÏóºÍ½áÂÛ¡£Response surface methodology, based on Box¨CBehnken design, is used to optimize the ultrasonic extraction conditions of Flammulina velutipes polysaccharides (FVP). Four independent variables (ratio of water to raw material, ultrasonic power, ultrasonic time, and ultrasonic temperature) are taken into consideration. Statistical analysis of the experiment indicates that the quadratic model fitted for the extraction conditions of FVP is in good agreement with the experimental results. A yield of FVP of 39.215% is obtained under the modified conditions (ratio of water to material 24 mL/g, ultrasonic power of 410 W, ultrasonic time of 25 min, and ultrasonic temperature of 35¡ãC). DPPH scavenging rate of 82.12%, AChE inhibitory rate of 18.51% obtained at 0.02g/mL in DPPH scavenging assay and AChE inhibitory activity test indicate a good potential of FVP to enhance cognitive ability.
3Â¥2010-07-09 10:02:25
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IASCC

Òø³æ (ÕýʽдÊÖ)

ÒÁÈËЦ(½ð±Ò+5, ·­ÒëEPI+1):·Ç³£¸Ðл£¬¸ø5¸ö°É£¬Ï´ÎÔÚ°ï°ïÎÒ°¡£¬ºÇºÇ 2010-07-09 16:11:14
Response surface methodology based on Box¨CBehnken design was used to optimize the ultrasonic extraction conditions of Flammulina velutipes polysaccharides (FVP). Four independent variables (ratio of water to raw material, ultrasonic power, ultrasonic time, and ultrasonic temperature) were considered.The rusults of statistical analysis indicated that the quadratic model fitted for the extraction conditions of FVP were very consistent with the experimental results. A yield of FVP of 39.215% was obtained under the modified conditions (ratio of water to material 24 mL/g, ultrasonic power of 410 W, ultrasonic time of 25 min, and ultrasonic temperature of 35¡ãC). DPPH scavenging rate of 82.12% and AChE inhibitory rate of 18.51% obtained at 0.02g/mL in DPPH scavenging assay and AChE inhibitory activity test indicated a good potential of FVP to enhance cognitive ability.
2Â¥2010-07-09 09:53:31
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