| 查看: 337 | 回复: 0 | |||
liushuai3788新虫 (初入文坛)
|
[交流]
【求助/交流】求关于rt-pcr的翻译
|
|
这一段是在看不懂了 只好来求助了 Serial 10-fold dilutions of GI and GII reference strains were amplified in triplicate and in parallel.The dilution curveswere linear over 5 and 6 orders of magnitude for the GI and GII real time assays,respectively.The slopes and the regression coefficients indicated good amplification efficiency.Fig.1 shows that the S35 strain titer was 10-fold higher than the Norwalk strain titer,but both were detected at 1 RT-PCR unit by the real-time RT-PCR assays with a mean Ct value of 42.4 and 40.1,respectively.They were both negative at the 0.1 RT-PCR U.The reproducibility was evaluated by testing serial dilutions of the positive control on ten separate runs.For the Norwalk strain,the variability was 2.8%for detection of 10 RT-PCR U(Ct values from 37.62 to 38.71)and for the GII S35 strain,the variability was 2.4%for detection of 10 RT-PCR U(Ct values from 38.84 to 39.79). |
回复此楼» 猜你喜欢
His标签蛋白纯化怎么才能快？
已经有0人回复
-
WRN变构抑制剂HRO761：从D1/D2界面锁定到MSI合成致死的工具化合物深度解析
已经有0人回复
-
化学工程及工业化学论文润色/翻译怎么收费?
已经有73人回复
-
KRAS G12V(ON) 选择性抑制剂 RMC-5127：从三元复合物机制到科研应用
已经有0人回复
-
SHP2 变构抑制剂 Sitneprotafib：从作用机制到科研试剂选型要点
已经有0人回复
-
PRMT5·MTA 抑制剂 MRTX1719 的科研应用
已经有0人回复
-
SW033291 科研使用解析：分子特征、储存与实验获取要点
已经有0人回复
-
泛 RAS 抑制新思路：大环肽拟似物如何同时牵住突变型与野生型 RAS？
已经有0人回复
-
TYK2/JAK1 双靶点工具化合物 Brepocitinib：激酶选择性、研究进展与实验应用全解析
已经有0人回复
-
矢车菊素-3-O-葡萄糖苷质控实录：从一张HPLC图谱读懂纯度与稳定性
已经有0人回复
-
恩诺沙星（Enrofloxacin）科研解析：分子特征、LC-MS/MS 检测与实验室使用要点
已经有0人回复
