一、招生方向
招生方向: 欢迎表观遗传学和生物信息学的考生联系调剂事宜
招生专业(学科) 0710J1生物育种 (调剂学科 0710生物学)
086001 生物技术与工程 (调剂学科0860生物与医药; 0836生物工程; 0831 生物医学工程; 0812计算机科学与技术; 0835软件工程)
请直接发邮件到: lfgu@fafu.edu.cn 不要直接在论坛回复
二、课题组简介及联系方式
顾连峰现为林学中心全职教授,博士生导师。2012年获得中国科学院生物信息学博士学位,2015年从美国加州大学河滨分校回国,2016年入选福建省C类引进高层次人才,2017年获第十四届林业青年科技奖获得者。现为福建农林大学“金山学者”。先后主持多项基金项目,包括国家自然基金面上项目三项、重点研发子课题三项。始终以表观遗传学为主线,以福建种植面积较广的禾本科材料为主要研究对象,解析快速生长和胁迫应答的表观遗传调控机制。共发表六十多篇SCI论文。有多篇通讯作者SCI论文在Genome Biology、Plant Physiology、Tree Physiology、Plant Journal、Bioinformatics和Journal of Integrative Plant Biology等期刊发表。
联系方式: lfgu@fafu.edu.cn
网站: https://teacher.fafu.edu.cn/hxlh/glf1/list.htm
三、研究方向及代表性成果
表观遗传学和生物信息学。长期从事表观遗传学相关研究,以植物为研究对象,结合多组学数据和分子生物学等手段,解析RNA修饰、DNA甲基化以及选择性拼接对快速生长和开花以及胁迫应答调控。建立了高效便捷的功能基因研究体系(Journal of Integrative Plant Biology,2023; Journal of Plant Biology,2021;Genomics, Proteomics & Bioinformatics,2023)。并解析非编码RNA 及N6-甲基腺嘌呤(m6A)在快速生长中的作用 (Plant physiology, 2022; JIPB, 2020; Tree physiology, 2022), 推进了生长发育的表观遗传学调控研究。建立了参考基因组序列(JIPB, 2022; 2024)。从单碱基水平定量解析N6-甲基腺嘌呤(m6A)修饰次生生长的调控 (Genome Biology, 2021; Plant physiology, 2022),解析DNA甲基化对开花和木质部发育的调控 (Plant Journal, 2021; Plant Journal, 2018; Plant Journal, 2023)。发表环状RNA和选择性拼接对生长发育调控(Plant Journal,2017;Cells, 2019; BMC plant biology, 2018; Frontiers in Genetics, 2019;Frontiers in Plant Science, 2021); 揭示环状RNA通过R-loop调控宿主基因的选择性拼接(JIPB,2021)并受转录后调控 (Bioinformatics, 2019);发现m6A和环状RNA和在快速生长中具有调控作用(Plant and Cell Physiology, 2019)。
部分发表论文:
Yandong Jin; Baijie Wang; Mingchuan Bao; Yujie Li; Shengwu Xiao; Yuhua Wang; Jun Zhang; Liangzhen Zhao; Hangxiao Zhang; Yau-Heiu Hsu; Mingjie Li; Lianfeng Gu. (2023). Development of an efficient expression system with large cargo capacity for interrogation of gene function in bamboo based on bamboo mosaic virus, Journal of Integrative Plant Biology, 65(6): 1369-1382
Yuxiang Liufu, Feihu Xi, Lin Wu, Zeyu Zhang, Huihui Wang, Huiyuan Wang, Jun Zhang, Baijie Wang, Wenjing Kou, Jian Gao, Liangzhen Zhao, Hangxiao Zhang, Lianfeng Gu. (2023). Inhibition of DNA and RNA methylation disturbs root development of moso bamboo. Tree Physiology, 43(9), pp.1653-1674.
Yongsheng Wang; Huihui Wang; Huiyuan Wang; Ruifan Zhou; Ji Wu; Zekun Zhang; Yandong Jin; Tao Li; Markus V. Kohnen; Xuqing Liu; Wentao Wei; Kai Chen; Yubang Gao; Jiazhi Ding; Hangxiao Zhang; Bo Liu; Chentao Lin; Lianfeng Gu. (2023). Multi-omics of circular RNAs and their responses to hormones in Moso bamboo (Phyllostachys edulis), Genomics, Proteomics & Bioinformatics, 21(4): 866-885
Yong Zhang; Yongcheng Wei; Jingxiang Meng; Yujiao Wang; Sen Nie; Zeyu Zhang; Huiyuan Wang; Yongkang Yang; Yubang Gao; Ji Wu; Tuhe Li; Xuqing Liu; Hangxiao Zhang; Lianfeng Gu. (2023) Chromosome-scale de novo genome assembly and annotation of three representative Casuarina species: C. equisetifolia, C. glauca, and C. cunninghamiana. Plant Journal. 114(6), pp.1490-1505.
Gao,Y., Liu, X., Wu, B., Wang, H., Xi, F., Kohnen, M., Reddy, A., Gu, L. (2021). Quantitative profiling of N6-methyladenosine at single-base resolution in stem-differentiating xylem of Populus trichocarpa using Nanopore direct RNA sequencing. Genome Biology. 22(1), 1-17.
Yubang Gao, Xuqing Liu, Yandong Jin, Ji Wu, Shuang Li, Yaxing Li, Binqing Chen, Yaxin Zhang, Linxiao Wei, Wei Li, Ruili Li, Chentao Lin, Anireddy S N Reddy, Pankaj Jaiswal, Lianfeng Gu. (2022). Drought induces epitranscriptome and proteome changes in stem-differentiating xylem of Populus trichocarpa. Plant Physiology, 190(1), 459-479
Wang,Y., Wang, H., Xi, F., Wang, H., Hang, X., Wei W., Zhang, H., Zhang, Q., Zheng, Y., Zhu, Q., Kohnen, M., Reddy, A., Gu, L. (2020). Profiling of circular RNA N6-methyladenosine in moso bamboo (Phyllostachys edulis) using nanopore-based direct RNA sequencing. Journal of Integrative Plant Biology, 62(12), 1823-1838.
Tao Li Huihui Wang, Yaxin Zhang, Huiyuan Wang, Zeyu Zhang, Xuqing Liu, Zekun Zhang, Kai Liu, Deming Yang, Hangxiao Zhang, Lianfeng Gu. (2022). Comprehensive profiling of epigenetic modifications in fast-growing moso bamboo shoots. Plant Physiology. https://doi.org/10.1093/plphys/kiac525.
Zhang, Z., Wang, H., Wang, Y., Xi, F., Wang, H., Kohnen, M., Gao, P., Wei, W., Chen, K., Liu, X., Gao, Y., Han, X., Hu, K., Zhang, H., Zhu, Q., Zheng, Y., Liu, B., Ahmad, A., Hsu, Y., Jacobsen, SE., Gu, L. (2021). Whole genome characterization of chronological age-associated changes in methylome and circular RNAs in moso bamboo (Phyllostachys edulis) from vegetative to floral growth, Plant Journal. 106(2), 435-453.
Ye, G., Zhang, H., Chen, B., Nie, S., Liu, H., Gao, W., Wang, H., Gao, Y. and Gu, L. (2018) De novo genome assembly of the stress tolerant forest species Casuarina equisetifolia provides insight into secondary growth. Plant Journal, 97(4), 779-794.
Yaxing Zhang, Jun Zhang, Deming Yang, Yandong Jin, Xuqing Liu, Zeyu Zhang, Lianfeng Gu, Hangxiao Zhang, 2022. Genome-wide identification of NAP1 and function analysis in moso bamboo (Phyllostachys edulis). International Journal of Molecular Sciences. 23(12), 6491
Liu, X., Gao, Y., Liao, J., Miao, M., Chen, K., Xi, F., Wei, W., Wang, H., Wang, Y., Xu, X., Reddy, A., Gu, L. (2021). Genome-wide profiling of circular RNAs, alternative splicing, and R-loops in stem-differentiating xylem of Populus trichocarpa. Journal of Integrative Plant Biology. 63(7), 1294-1308.
Wang, HY., Wang, HH., Zhang, H., Liu, S., Wang, Y., Gao, Y., Xi, F., Zhao, L., Liu, B., Reddy, A., Lin, C., Gu, L. (2019). The interplay between microRNA and alternative splicing of linear and circular RNAs in eleven plant species. Bioinformatics, 35(17), 3119-3126.
Wang, Y., Gao, Y., Zhang, H., Wang, H., Liu, X., Xu, X., Zhang, Z., Kohnen, M., Hu, K., Wang, H., Xi, F., Zhao, L., Lin, C., Gu, L. (2019). Genome-wide profiling of circular RNAs in the rapidly growing shoots of moso bamboo (Phyllostachys edulis). Plant and Cell Physiology, 60(6), 1354-1373.
Wang, T., Wang, H., Cai, D., Gao, Y., Zhang, H., Wang, Y., Lin, C., Ma, L. and Gu, L. (2017) Comprehensive profiling of rhizome-associated alternative splicing and alternative polyadenylation in moso bamboo (Phyllostachys edulis). Plant Journal, 91, 684-699.
Yu, X., Wang, Y., Kohnen, M. V., Piao, M., Tu, M., Gao, Y., Lin, C., Zuo, Z., & Gu, L. (2019). Large scale profiling of protein isoforms using label-free quantitative proteomics revealed the regulation of nonsense-mediated decay in moso bamboo (Phyllostachys edulis). Cells, 8(7), 744.
Gao, Y., Zhang, H., Wang, Y., Chen, J. and Gu, L. (2018) PRAPI: post-transcriptional regulation analysis pipeline for Iso-Seq. Bioinformatics, 34, 1580-1582.
Zhao, L., Zhang, H., Kohnen, M., Prasad, K., Gu, L., Reddy, A. S. (2019). Analysis of transcriptome and epitranscriptome in plants using PacBio Iso-Seq and nanopore-based direct RNA sequencing. Frontiers in Genetics, 10, 253.
Zhang, H., Wang, H., Zhu, Q., Gao, Y., Wang, H., Zhao, L.,Wang, Y., Xi, F., Wang, W.,Yang, Y., Lin, C., Gu, L. (2018), Transcriptome characterization of moso bamboo (Phyllostachys edulis) seedlings in response to exogenous gibberellin applications, BMC plant biology, 2018,18:125.
Zhang H, Lin C, Gu L (2017). Light regulation of alternative Pre-mRNA splicing in plants. Photochemistry and Photobiology, 93(1), 159-165.
Chen, K., Hu, K., Xi, F., Wang, H., Kohnen, M., Gao, P., Liao, J., Wei, W., Liu, X., Zhang, H., Gu, L. (2021). High-efficient and transient transformation of Moso Bamboo (Phyllostachys edulis) and Ma Bamboo (Dendrocalamus latiflorus Munro). Journal of Plant Biology, 91(4), 684-699.
Wang, H., Liu, S., Dai, X., Yang, Y., Luo, Y., Gao, Y., Liu, X., Wei, W., Wang, H., Xu, X., Reddy, A., Jaiswal., P. Li, W., Liu, B., Gu, L. (2021). PSDX: A comprehensive multi-omics association database of Populus trichocarpa with a focus on the secondary growth in response to stresses. Front. Plant Sci, doi: 10.3389/fpls.2021.655565.
Wentao Wei, Huiyuan Wang, Xuqing Liu, Wenjing Kou, Ziqi Liu, Huihui Wang, Yongkang Yang, Liangzhen Zhao, Hangxiao Zhang, Bo Liu, Xiangqing Ma, Lianfeng Gu. 2022. Transcriptome Profiling of Stem-Differentiating Xylem in Response to Abiotic Stresses Based on Hybrid Sequencing in Cunninghamia lanceolata. International Journal of Molecular Sciences, 23(22), 13986
Zekun Zhang, Huiyuan Wang, Ji Wu, Yandong Jin, Shengwu Xiao, Tao Li, Xuqinq Liu,Hangxiao Zhang, Zeyu Zhang, Jun Su, Jingzao Liu, Xiaoyan Wang, Yubang Gao, Xiangqing Ma and Lianfeng Gu. (2022). Comprehensive Transcriptome Analysis of Stem-Differentiating Xylem Upon Compression Stress in Cunninghamia Lanceolata." Frontiers in genetics 13.
Liufu, Y., Liu, X., Wu, L., Zhang, H., Gao, Y. and Gu, L. (2022). An Analysis Pipeline for Identification of RNA Modification, Alternative Splicing and Polyadenylation Using Third Generation Sequencing. Bio-101: e4433. DOI: 10.21769/BioProtoc.4433.
Yushan Zheng, Deming Yang, Jundong Rong, Liguang Chen, Qiang Zhu, Tianyou He, Lingyan Chen, Jing Ye, Lili Fan, Yubang Gao, Hangxiao Zhang, Lianfeng Gu. (2022). Allele-aware chromosome-scale assembly of the allopolyploid genome of hexaploid Ma Bamboo (Dendrocalamus latiflorus Munro) J Integr Plant Biol., 64(3), 649-670. |