²é¿´: 452  |  »Ø¸´: 4
µ±Ç°Ö÷ÌâÒѾ­´æµµ¡£
µ±Ç°Ö»ÏÔʾÂú×ãÖ¸¶¨Ìõ¼þµÄ»ØÌû£¬µã»÷ÕâÀï²é¿´±¾»°ÌâµÄËùÓлØÌû

xiaoweiwei8033

Í­³æ (СÓÐÃûÆø)

[½»Á÷] ¹ØÓÚÀë×Ó½»»»²ãÎöÖù£¿

Àë×Ó½»»»²ãÎöÖùÓÐºÜ¶à¹æ¸ñµÄ°É£¿
Column PD-10, Sephadex G25M Columns, Pharmacia Biotech
ÊÇʲôÒâ˼£¬Ê²Ã´¹æ¸ñµÄÄØ£¿
Çë¸ßÊÖ½â»ó£¡£¡~
»Ø¸´´ËÂ¥

» ²ÂÄãϲ»¶

ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

linruxin

½ð³æ (СÓÐÃûÆø)

¡ï
Сľ³æ(½ð±Ò+0.5):¸ø¸öºì°ü£¬Ð»Ð»»ØÌû½»Á÷
columns PD-10 ÊÇԤװµÄG-25ÍÑÑÎÖù£¬ÊôÓÚ·Ö×ÓɸÀ࣬·ÇÀë×Ó½»»»Àà¡£

µ±È»Àë×Ó½»»»ÌîÁÏÓкܶàÖÖÀàÐÍ£º±ÈÈçÓи÷¸ö³§¼ÒÓõĻùÖÊ¿ÉÄܲ»Í¬£¬µ«×îºó»¹Êǹé½áΪǿÑô£¬ÈõÑô£¬Ç¿Òõ£¬ÈõÒõ4ÖÖ£¬ÆäÖÐÇ¿ºÍÈõÖ»Êǹ¤×÷pH·¶Î§²»Í¬£¬¸ú½áºÏÀι̶̳ȣ¬ÔØÁ¿¶àÉÙûÓбØÈ»ÁªÏµ¡£
3Â¥2009-09-11 14:23:44
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû
²é¿´È«²¿ 5 ¸ö»Ø´ð

linruxin

½ð³æ (СÓÐÃûÆø)

¡ï
Сľ³æ(½ð±Ò+0.5):¸ø¸öºì°ü£¬Ð»Ð»»ØÌû½»Á÷
Amersham Pharmacia columns PD-10 are prepacked,disposable columns containing Sephadex® G-25 M for rapid desalting and buffer exchange.

Dimensions
Bed volume: 9.1 ml
Bed height: 5 cm

Eluent composition
Columns PD-10 contain Sephadex G-25 M swollen indistilled water containing 0.15% Kathon® CG/ICP
Biocide* as preservative. The columns can be rapidly equilibrated with other aqueous buffer solutions.


Instructions for use
1. Remove the top cap and pour off the excess liquid.
2. Remove the bottom cap.
3. Support the column over a suitable receptacle and equilibrate the gel bed with approximately 25 ml of the required buffer solution.
4. When the equilibration buffer has run into the column, add the sample in a total volume of 2.5 ml. If the sample volume is less than 2.5 ml, add buffer until sample volume plus buffer volume 2.5 ml. Discard the eluent.
5. When the sample has run into the column, elute the high molecular weight components e.g. proteins, with 3.5 ml elution buffer solution.


Note
Small air bubbles along the plastic walls of the column and on the bottom membran may occur. This does not affect the performance of the column.

[ Last edited by linruxin on 2009-9-11 at 12:11 ]
2Â¥2009-09-11 12:05:13
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

xiaoweiwei8033

Í­³æ (СÓÐÃûÆø)

As much as possible of the supernatant (about 0.9 ml) was pipetted on to a cation exchange column (Column PD-10, Sephadex G25M Columns, Pharmacia Biotech)¡£
ÎÒÊÇÔÚÎÄÏ×µÄÕâ¾ä»°ÉÏ¿´¼ûµÄ£¬¡°a cation exchange column ¡±Õâ¸ö·­Òë¹ýÀ´²»¾ÍÊÇÀë×Ó½»»»ÖùÂð£¿
5Â¥2009-09-11 14:38:23
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû
×î¾ßÈËÆøÈÈÌûÍÆ¼ö [²é¿´È«²¿] ×÷Õß »Ø/¿´ ×îºó·¢±í
[¿¼ÑÐ] 311Çóµ÷¼Á +3 ¶¬Ê®Èý 2026-03-24 3/150 2026-03-24 21:31 by peike
[¿¼ÑÐ] Ò»Ö¾Ô¸±±»¯315 Çóµ÷¼Á +3 akrrain 2026-03-24 3/150 2026-03-24 19:35 by ÁËÁËÁËÁË¡£¡£
[¿¼ÑÐ] 292Çóµ÷¼Á +4 ¶ì¶ì¶ì¶î¶î¶î¶î¶ 2026-03-24 4/200 2026-03-24 16:41 by peike
[¿¼ÑÐ] Ò»Ö¾Ô¸211 ³õÊÔ270·Ö Çóµ÷¼Á +5 ¹ÈÓêÉϰ¶ 2026-03-23 6/300 2026-03-24 16:32 by laoshidan
[¿¼ÑÐ] Ò»Ö¾Ô¸±±¾©»¯¹¤´óѧ²ÄÁÏÓ뻯¹¤ 264·Ö¸÷¿Æ¹ýAÇø¹ú¼ÒÏß +3 ¹þ¹þ157349 2026-03-21 3/150 2026-03-24 14:11 by zhyzzh
[¿¼ÑÐ] 284Çóµ÷¼Á +10 Zhao anqi 2026-03-22 10/500 2026-03-24 00:08 by Equinoxhua
[¿¼ÑÐ] 335·Ö | ²ÄÁÏÓ뻯¹¤×¨Ë¶ | GPA 4.07 | ÓпÆÑо­Àú +4 cccchenso 2026-03-23 4/200 2026-03-23 23:00 by Ðìckkk
[¿¼ÑÐ] 284Çóµ÷¼Á +3 yanzhixue111 2026-03-23 6/300 2026-03-23 22:58 by pswait
[¿¼ÑÐ] Ò»Ö¾Ô¸ÉÂʦ´óÉúÎïѧ071000£¬298·Ö£¬Çóµ÷¼Á +3 SYA£¡ 2026-03-23 3/150 2026-03-23 19:09 by macy2011
[¿¼ÑÐ] 336Çóµ÷¼Á +4 ÊÕµ½VS 2026-03-20 4/200 2026-03-23 19:02 by macy2011
[¿¼ÑÐ] 316Çóµ÷¼Á +7 ÁºÜçö© 2026-03-19 7/350 2026-03-23 16:21 by lingjue
[¿¼ÑÐ] ÇóÀÏʦÊÕÎÒ +3 zzh16938784 2026-03-23 3/150 2026-03-23 12:56 by ztnimte
[¿¼ÑÐ] Çóµ÷¼Á²ÄÁÏѧ˶080500£¬×Ü·Ö289·Ö 5+3 @taotao 2026-03-19 21/1050 2026-03-23 10:17 by ¹Úc¸ç
[¿¼ÑÐ] Ò»Ö¾Ô¸»ªÖÐũҵ071010£¬×Ü·Ö320Çóµ÷¼Á +5 À§À§À§À§À¤À¤ 2026-03-20 6/300 2026-03-22 17:41 by hxsm
[¿¼ÑÐ] 303Çóµ÷¼Á +5 °²ÒäÁé 2026-03-22 6/300 2026-03-22 12:46 by ËØÑÕÇã³Ç1988
[¿¼ÑÐ] 085600²ÄÁÏÓ뻯¹¤306 +4 z1z2z3879 2026-03-21 4/200 2026-03-21 23:44 by ms629
[¿¼ÑÐ] 336Çóµ÷¼Á +5 rmc8866 2026-03-21 5/250 2026-03-21 17:24 by ѧԱ8dgXkO
[¿¼ÑÐ] 299Çóµ÷¼Á +5 shxchem 2026-03-20 7/350 2026-03-21 17:09 by ColorlessPI
[¿¼ÑÐ] Ò»Ö¾Ô¸»ªÖпƼ¼´óѧ£¬080502£¬354·ÖÇóµ÷¼Á +5 ÊØºòϦÑôCF 2026-03-18 5/250 2026-03-21 01:06 by JourneyLucky
[¿¼ÑÐ] 295¸´ÊÔµ÷¼Á +8 ¼òľChuFront 2026-03-19 8/400 2026-03-20 20:44 by zhukairuo
ÐÅÏ¢Ìáʾ
ÇëÌî´¦ÀíÒâ¼û