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xiaoweiwei8033

铜虫 (小有名气)

[交流] 关于离子交换层析柱?

离子交换层析柱有很多规格的吧?
Column PD-10, Sephadex G25M Columns, Pharmacia Biotech
是什么意思,什么规格的呢?
请高手解惑!!~
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linruxin

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Amersham Pharmacia columns PD-10 are prepacked,disposable columns containing Sephadex® G-25 M for rapid desalting and buffer exchange.

Dimensions
Bed volume: 9.1 ml
Bed height: 5 cm

Eluent composition
Columns PD-10 contain Sephadex G-25 M swollen indistilled water containing 0.15% Kathon® CG/ICP
Biocide* as preservative. The columns can be rapidly equilibrated with other aqueous buffer solutions.


Instructions for use
1. Remove the top cap and pour off the excess liquid.
2. Remove the bottom cap.
3. Support the column over a suitable receptacle and equilibrate the gel bed with approximately 25 ml of the required buffer solution.
4. When the equilibration buffer has run into the column, add the sample in a total volume of 2.5 ml. If the sample volume is less than 2.5 ml, add buffer until sample volume plus buffer volume 2.5 ml. Discard the eluent.
5. When the sample has run into the column, elute the high molecular weight components e.g. proteins, with 3.5 ml elution buffer solution.


Note
Small air bubbles along the plastic walls of the column and on the bottom membran may occur. This does not affect the performance of the column.

[ Last edited by linruxin on 2009-9-11 at 12:11 ]
2楼2009-09-11 12:05:13
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linruxin

金虫 (小有名气)


小木虫(金币+0.5):给个红包,谢谢回帖交流
columns PD-10 是预装的G-25脱盐柱,属于分子筛类,非离子交换类。

当然离子交换填料有很多种类型:比如有各个厂家用的基质可能不同,但最后还是归结为强阳,弱阳,强阴,弱阴4种,其中强和弱只是工作pH范围不同,跟结合牢固程度,载量多少没有必然联系。
3楼2009-09-11 14:23:44
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xiaoweiwei8033

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谢谢啦!
4楼2009-09-11 14:33:56
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xiaoweiwei8033

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As much as possible of the supernatant (about 0.9 ml) was pipetted on to a cation exchange column (Column PD-10, Sephadex G25M Columns, Pharmacia Biotech)。
我是在文献的这句话上看见的,“a cation exchange column ”这个翻译过来不就是离子交换柱吗?
5楼2009-09-11 14:38:23
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