| 查看: 505 | 回复: 4 | |||
| 当前主题已经存档。 | |||
xiaoweiwei8033铜虫 (小有名气)
|
[交流]
关于离子交换层析柱？
|
||
|
离子交换层析柱有很多规格的吧？ Column PD-10, Sephadex G25M Columns, Pharmacia Biotech 是什么意思，什么规格的呢？ 请高手解惑！！~ |
回复此楼» 猜你喜欢
2026年面上项目中了，2A+B, 会评顺利通过
已经有5人回复
-
售T0P一区SCI文章，我:8O5.51.O.54,科目齐全,可＋急
已经有4人回复
-
售T0P一区SCI文章，我:8O5.51.O.54,科目齐全,可＋急
已经有4人回复
-
大家好，校样时候的紧急求助，请各位帮帮忙了
已经有7人回复
-
昨日死，今日生
已经有7人回复
-
售T0P一区SCI文章，我:8O5.51.O.54,科目齐全,可＋急
已经有7人回复
-
植酸TLC薄层色谱爬板
已经有6人回复
linruxin
金虫 (小有名气)
- 应助: 0 (幼儿园)
- 金币: 770.1
- 帖子: 133
- 在线: 10.3小时
- 虫号: 639065
- 注册: 2008-10-28
- 性别: GG
- 专业: 合成药物化学
★
小木虫(金币+0.5):给个红包，谢谢回帖交流
小木虫(金币+0.5):给个红包，谢谢回帖交流
|
Amersham Pharmacia columns PD-10 are prepacked,disposable columns containing Sephadex® G-25 M for rapid desalting and buffer exchange. Dimensions Bed volume: 9.1 ml Bed height: 5 cm Eluent composition Columns PD-10 contain Sephadex G-25 M swollen indistilled water containing 0.15% Kathon® CG/ICP Biocide* as preservative. The columns can be rapidly equilibrated with other aqueous buffer solutions. Instructions for use 1. Remove the top cap and pour off the excess liquid. 2. Remove the bottom cap. 3. Support the column over a suitable receptacle and equilibrate the gel bed with approximately 25 ml of the required buffer solution. 4. When the equilibration buffer has run into the column, add the sample in a total volume of 2.5 ml. If the sample volume is less than 2.5 ml, add buffer until sample volume plus buffer volume 2.5 ml. Discard the eluent. 5. When the sample has run into the column, elute the high molecular weight components e.g. proteins, with 3.5 ml elution buffer solution. Note Small air bubbles along the plastic walls of the column and on the bottom membran may occur. This does not affect the performance of the column. [ Last edited by linruxin on 2009-9-11 at 12:11 ] |
2楼2009-09-11 12:05:13
linruxin
金虫 (小有名气)
- 应助: 0 (幼儿园)
- 金币: 770.1
- 帖子: 133
- 在线: 10.3小时
- 虫号: 639065
- 注册: 2008-10-28
- 性别: GG
- 专业: 合成药物化学
3楼2009-09-11 14:23:44
xiaoweiwei8033
铜虫 (小有名气)
- 应助: 0 (幼儿园)
- 金币: 81.4
- 帖子: 67
- 在线: 11分钟
- 虫号: 668806
- 注册: 2008-12-05
- 性别: MM
- 专业: 植物生理
4楼2009-09-11 14:33:56
xiaoweiwei8033
铜虫 (小有名气)
- 应助: 0 (幼儿园)
- 金币: 81.4
- 帖子: 67
- 在线: 11分钟
- 虫号: 668806
- 注册: 2008-12-05
- 性别: MM
- 专业: 植物生理
5楼2009-09-11 14:38:23