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求助中翻英段落
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| 参考李合生等人的方法，取10ml藻液，在室温下，用布氏漏斗将混合液抽滤，抽滤后的滤纸放入25ml的比色管中，然后加入10ml 95%的酒精萃取，放于冰箱在4摄氏度条件下的暗处静置24小时，然后再将溶液在4000r/min的条件下离心15分钟，取上清液用分光光度计分别在665，649，470nm处测定溶液的吸光度，采用1cm的比色皿，用95％的乙醇做空白， |
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jw227
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玉米庄主(金币+6,VIP+0):thanks 9-10 09:50
玉米庄主(金币+6,VIP+0):thanks 9-10 09:50
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Referring to the method of Li He-Sheng and others，10ml algal mixture was filtrated with air pump at room temperature in a Büchner funnel. Filter paper of the filtrate was extracted using ethanol (10ml, 95%) in colorimetric tube. After standing at the refrigerator in the dark for 24h, the solution was centrifuged for 15 minutes at 4000r/min. The supernatant was analyzed at 665,649,470 nm in colorimetric ware (1 cm) by the spectrophotometer and 95% ethanol was used as a blank. [ Last edited by jw227 on 2009-9-10 at 09:30 ] |
3楼2009-09-10 09:28:39
| Li He-Sheng and others refer to the method, take 10ml algal liquid at room temperature with a Büchner funnel to the mixture leaching, leaching into the post-filter paper 25ml colorimetric tube, then add 10ml 95% alcohol extraction, put in refrigerator at 4 degrees Celsius under the conditions of the dark put it aside for 24 hours, and then under the conditions of solution at 4000r/min centrifuged for 15 minutes, then centrifuged at 665,649,470 nm with the spectrophotometer was measured solution absorbance, using 1cm cuvette, with 95% ethanol to do blank, cortical regions emerge in adulthood from the reversed pattern of childhood cortical asymmetries |
2楼2009-09-10 08:42:36
4楼2009-09-10 09:50:01