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During the fabrication process, the microspheres were collected by centrifugation, and the supernatant was collected. The BCA protein assay was utilized to determine the concentration of BSA present in the supernatant, thus providing the mass of protein not encapsulated. A mass balance was performed to determine the amount of BSA that was loaded into the microspheres. The protein that was lost while washing the microspheres during the fabrication process was not accounted for when calculating the loading. Hence the loading and loading efficiency are slightly overestimated. The encapsulation efficiency of the protein was determined by dividing the mass of the loaded protein by the initial mass of protein. The entrapment could be determined in a reproducible and quantitative manner.

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yuyang031985

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Originally posted by rain841230 at 2009-9-4 22:25:
In the synthesis procedure, the micocapsules were obtained by centrifuating the suspension, and the supernatant was also collected for further characterizations. Firstly, the amount of protein that ...

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rain841230

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yuyang031985(½ð±Ò+10,VIP+0):·Ç³£¸Ðл£¡ 9-5 20:02
In the synthesis procedure, the micocapsules were obtained by centrifuating the suspension, and the supernatant was also collected for further characterizations. Firstly, the amount of protein that was not encapsulated was known by the BSA content in the supernatant, which could be determined by means of BCA protein assay. Then, the amount of encapsulated BSA in the microspheres, therefore, could be easily calculated by mass balancing, during which the amount of protein that lost while leaching was not considered. On that account, the loading contents of the protein were overestimated with a very small deviation. The encapsulation efficiency of the protein was worked out by calculating the ratio of the mass of the encapsulated protein to the overall mass of the protein that utilized in the synthesis. ×îºóÒ»¾äµÄentrapmentÊÇʲô£¿£¿

[ Last edited by rain841230 on 2009-9-4 at 22:32 ]
2Â¥2009-09-04 22:25:20
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