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Apply to the plate 10 ¦Ìl of test solution (a) and 5 ¦Ìl each of test solution (b), reference solution (a), reference solution (b) and reference solution (c).Develop over a path of 10 cm using a mixture of 15 volumes of glacial acetic acid R, 25 volumes of water R and 60 volumes of butanol R. Allow the plate to dry in air.Spray the plate with a 5 g/l solution of dimethylaminobenzaldehyde R in a mixture of 1 volume of hydrochloric acid R and 3 volumes of methanol R. Dry the plate in a current of hot air.Examine in daylight after 30 min. Any spot in the chromatogram obtained with test solution (a), apart from the principal spot,£¬ is not more intense than the spot in the chromatogram obtained with reference solution (b) (0.5 per cent). The test is not valid unless the chromatogram obtained with reference solution (c) shows two clearly separated principal spots.

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xiaoyangcheng(½ð±Ò+1,VIP+0):лл 9-3 17:49
The test is not valid unless the chromatogram obtained with reference solution (c) shows two clearly separated principal spots.
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Originally posted by xiaoyangcheng at 2009-9-2 15:22:
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