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xiaoyangcheng

金虫 (正式写手)


[交流] 一段化学色谱分析方法的翻译

Apply to the plate 10 μl of test solution (a) and 5 μl each of test solution (b), reference solution (a), reference solution (b) and reference solution (c).Develop over a path of 10 cm using a mixture of 15 volumes of glacial acetic acid R, 25 volumes of water R and 60 volumes of butanol R. Allow the plate to dry in air.Spray the plate with a 5 g/l solution of dimethylaminobenzaldehyde R in a mixture of 1 volume of hydrochloric acid R and 3 volumes of methanol R. Dry the plate in a current of hot air.Examine in daylight after 30 min. Any spot in the chromatogram obtained with test solution (a), apart from the principal spot,, is not more intense than the spot in the chromatogram obtained with reference solution (b) (0.5 per cent). The test is not valid unless the chromatogram obtained with reference solution (c) shows two clearly separated principal spots.

求助说明:这是EP上关于Allantoin的一段,除了翻译成汉语外,我还有两点疑问,一是文中的R代表什么,还是没什么实际意义,二是开始的那句是测试液a、b和参比液a、b、c同时还是分别点板?

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curtlee

金虫 (小有名气)

引用回帖:
Originally posted by xiaoyangcheng at 2009-9-2 15:22:
如果说R是代表色谱纯,那么文中water后面的R也是了,如果是,是不是经过微滤膜的水

[ Last edited by xiaoyangcheng on 2009-9-2 at 15:23 ]

应该是吧,不过水一般好像不这么叫,像我们跑柱子一般用比较好的屈臣氏蒸馏水
4楼2009-09-02 21:40:14
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curtlee

金虫 (小有名气)

R在这里指的是色谱纯,所有都点在一块板上。至于翻译,自己搞定吧,不难的,反正自己以后要经常用的
2楼2009-09-02 15:16:03
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xiaoyangcheng

金虫 (正式写手)


如果说R是代表色谱纯,那么文中water后面的R也是了,如果是,是不是经过微滤膜的水

[ Last edited by xiaoyangcheng on 2009-9-2 at 15:23 ]
3楼2009-09-02 15:22:14
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monitor2885

至尊木虫 (职业作家)

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xiaoyangcheng(金币+1,VIP+0):谢谢 9-3 17:49
The test is not valid unless the chromatogram obtained with reference solution (c) shows two clearly separated principal spots.
除非得到的色谱与参考溶液比较得到的2个主要点是明显分离的,否则测试结果将无效。
Retirement
5楼2009-09-02 22:20:08
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