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xuezhaoteng

金虫 (正式写手)

[求助] 请教时间分辨荧光的问题

The authors stated that the probe can eliminate the impact of auto-fluorescence effect due to the long-lived luminescence of Tb. However, the fluorescence recognition of the probe for Aβ in this work was measured by the steady-fluorescence mode rather than time-resolved luminescence mode. Accordingly, since the fluorescence signal was collected, which cannot reflect the ability of long-lived luminescence to eliminate the short-live background fluorescence. Thus, the comparison between steady-fluorescence and time-resolved luminescence for Aβ recognition should be provided.

上述为审稿人的问题,没怎么看明白如何测试 time-resolved luminescence for Aβ ,请大虫们赐教。
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kestrekq

金虫 (正式写手)

2楼2021-08-27 17:21:02
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好着呢

新虫 (小有名气)

用time-gated设备,调延迟时间测试跟稳态对比就行吧

发自小木虫Android客户端
3楼2021-08-27 20:33:50
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