Originally posted by smallcat227 at 2009-7-28 15:33:
楼主用普通的Taq扩增效果如何？

同意楼上几位说的，延长延伸时间，退火时间差不多够了

不过最根本的问题还是引物设计，有条件的话最好还是重新设计

Originally posted by ytetyio09 at 2009-7-28 15:20:
obviously, your extension time is too short, use 4-5min, have a try again. Because the speed of PFU amplification is about 500bp/min.

your annealing time is more than enough.

[ Last edited b ...

Originally posted by 381466632 at 2009-7-28 14:51:


首先谢谢你，可不可以把oligo看错配说得详细点，学习一下。还有我的扩增条带1900左右。退火时间需要延长吗？