| 查看: 252 | 回复: 4 | |||
| 当前主题已经存档。 | |||
| 当前只显示满足指定条件的回帖，点击这里查看本话题的所有回帖 | |||
[交流]
求租高人翻译一段
|
|||
|
求助高人帮忙翻译这段，谢谢！ To measure the glycerol content of the cells , the strains of C. albicans were cultured in a YPD medium at 28degree Centigrade for 2 days , and then the cell suspensions were incubated in fresh YPD medium with fungicides or 6%NaCL for 5h at 28degree Centigrade . After incubation, the cells were collected by centrifugation ,washed twice with distilled water , and then extracted at 80degree Centigrade for 5 min. After centrifugation at 15000rpm, the glycerol concentration in the supernatant was measured by the UV-glycerol assay prodedure (R-Biopharm GmbH) described previously . The same samples were dried for 24h at 85 degree Centigrade to measure their dry weight . |
回复此楼» 猜你喜欢
286求调剂
已经有14人回复
-
一志愿211，0703化学305分求调剂
已经有10人回复
-
362求调剂
已经有8人回复
-
0703调剂
已经有17人回复
-
333求调剂
已经有4人回复
-
085600材料与化工专硕329 求调剂
已经有12人回复
-
一志愿吉大化学327求调剂
已经有8人回复
-
0703化学调剂 348分
已经有8人回复
-
一志愿太原理工大学计算机技术专硕348，求调剂指导
已经有3人回复
-
材料考研求调剂总分280
已经有16人回复
3楼2009-07-27 10:24:27
★ ★ ★ ★ ★ ★
cwf128(金币+6,VIP+0):首先谢谢了，and then extracted at 80degree Centigrade for 5 min。extracted是指怎么提起，谢谢 7-27 14:51
cwf128(金币+6,VIP+0):首先谢谢了，and then extracted at 80degree Centigrade for 5 min。extracted是指怎么提起，谢谢 7-27 14:51
| 为了检测细胞中的甘油含量，将白念珠菌株置于于YPD培养基中以28摄氏度培养2天。然后将细胞悬浮物在新鲜YPD培养基，并加入杀菌剂或6 ％ NaCl溶液，28摄氏度恒温培养５小时。然后，将细胞离心，用蒸馏水清洗两次，然后在80摄氏度提取5分钟。经过15000rpm离心，甘油上清液用前述紫外线甘油法测定 （R-Biopharm GmbH公司）。同一样品在85摄氏度干燥24小时，称重。 |
2楼2009-07-27 00:14:36
4楼2009-07-27 14:51:47
5楼2009-07-27 19:29:26
