| 查看: 295 | 回复: 4 | |||
| 当前主题已经存档。 | |||
[交流]
求租高人翻译一段
|
|||
|
求助高人帮忙翻译这段，谢谢！ To measure the glycerol content of the cells , the strains of C. albicans were cultured in a YPD medium at 28degree Centigrade for 2 days , and then the cell suspensions were incubated in fresh YPD medium with fungicides or 6%NaCL for 5h at 28degree Centigrade . After incubation, the cells were collected by centrifugation ,washed twice with distilled water , and then extracted at 80degree Centigrade for 5 min. After centrifugation at 15000rpm, the glycerol concentration in the supernatant was measured by the UV-glycerol assay prodedure (R-Biopharm GmbH) described previously . The same samples were dried for 24h at 85 degree Centigrade to measure their dry weight . |
回复此楼» 猜你喜欢
生命口C13面上会评时间
已经有11人回复
-
内心匮乏
已经有16人回复
-
生命口面上会评结束了吗大家有知道结果吗？现在还没有结果正常不？
已经有9人回复
-
有机物中金属离子降到1ppm以下，有什么好的方法吗？用过有机溶剂重结晶还是降不下来
已经有4人回复
-
投英文期刊如何查重
已经有6人回复
-
不要再数国自然申请书的 filecode 的分隔符个数了
已经有21人回复
-
伯胺上甲基求助
已经有7人回复
-
跨出版社商投稿
已经有5人回复
-
5个%2F，非固定段22个字母
已经有7人回复
-
生生命学部会评
已经有4人回复
★ ★ ★ ★ ★ ★
cwf128(金币+6,VIP+0):首先谢谢了，and then extracted at 80degree Centigrade for 5 min。extracted是指怎么提起，谢谢 7-27 14:51
cwf128(金币+6,VIP+0):首先谢谢了，and then extracted at 80degree Centigrade for 5 min。extracted是指怎么提起，谢谢 7-27 14:51
| 为了检测细胞中的甘油含量，将白念珠菌株置于于YPD培养基中以28摄氏度培养2天。然后将细胞悬浮物在新鲜YPD培养基，并加入杀菌剂或6 ％ NaCl溶液，28摄氏度恒温培养５小时。然后，将细胞离心，用蒸馏水清洗两次，然后在80摄氏度提取5分钟。经过15000rpm离心，甘油上清液用前述紫外线甘油法测定 （R-Biopharm GmbH公司）。同一样品在85摄氏度干燥24小时，称重。 |
2楼2009-07-27 00:14:36
3楼2009-07-27 10:24:27
4楼2009-07-27 14:51:47
5楼2009-07-27 19:29:26