| 查看: 273 | 回复: 3 | |||
| 当前主题已经存档。 | |||
[交流]
帮忙翻译一小段摘要，谢谢
|
|||
| 摘要：将新城疫病毒（Newcastle disease virus, NDV）ND-xx08 株分离、纯化、培养后，提取其病毒的基因组 RNA，RT-PCR扩增获得与HN基因预期大小一致的 DNA片段，克隆到 pMD-18载体，经 EcoR I 和 Hind III 双酶切鉴定正确后送往上海生工测序。经测定，其 HN 基因的序列长度为 1 716bp,共编码 571 个氨基酸。与国内外发表的24株 NDV 相同序列的同源性分析表明，其核苷酸序列的同源性在80％－98.4％之间，氨基酸同源性在87.2％－98.2％之间。 |
回复此楼» 猜你喜欢
085600，321分求调剂
已经有11人回复
-
复试调剂
已经有8人回复
-
材料调剂
已经有12人回复
-
301求调剂
已经有11人回复
-
283求调剂
已经有10人回复
-
0703化学
已经有11人回复
-
322求调剂：一志愿湖南大学 材料与化工（085600），已过六级。
已经有18人回复
-
070300化学学硕311分求调剂
已经有11人回复
-
材料化工306分找合适调剂
已经有12人回复
-
298求调剂
已经有3人回复
今夜起风
金虫 (正式写手)
- 应助: 0 (幼儿园)
- 金币: 1115.1
- 散金: 218
- 帖子: 436
- 在线: 119.6小时
- 虫号: 641325
- 注册: 2008-10-30
- 性别: GG
- 专业: 无机纳米化学
★
jwk3029(金币+1):谢谢参与
jwk3029(金币+1,VIP+0): 7-26 19:44
qingfeng877680(金币-1,VIP+0):使用软件或网站翻译，请注明：【机器翻译】，谢谢！ 7-27 11:51
jwk3029(金币+1):谢谢参与
jwk3029(金币+1,VIP+0): 7-26 19:44
qingfeng877680(金币-1,VIP+0):使用软件或网站翻译，请注明：【机器翻译】，谢谢！ 7-27 11:51
| Abstract:To Newcastle disease virus (Newcastle disease virus, NDV) ND-xx08 line separation, purification, culture, the extraction of the virus genome RNA, RT-PCR amplification with the HN gene was consistent with the expected size of DNA fragments cloned into pMD-18 vector, the EcoR I and Hind III double digestion to identify correct sequencing of the Public Health and sent to Shanghai. By measuring its HN gene sequence length for the 1 716bp, encoding 571 amino acids. At home and abroad published the same 24 NDV sequence homology analysis showed that the nucleotide sequence homology of 80% -98.4%, and amino acid homology of 87.2% -98.2% in between. |
2楼2009-07-26 19:18:49
3楼2009-07-26 23:21:25
★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★
jwk3029(金币+1):谢谢参与
jwk3029(金币+21,VIP+0): 7-27 08:14
qingfeng877680(金币+1,VIP+0):谢谢兄弟指出二楼为机器翻译！ 7-27 11:52
jwk3029(金币+1):谢谢参与
jwk3029(金币+21,VIP+0): 7-27 08:14
qingfeng877680(金币+1,VIP+0):谢谢兄弟指出二楼为机器翻译！ 7-27 11:52
|
二楼那是机器翻译。 Abstract: ND-xx08 from Newcastle Disease Virus (NDV) was separated, purified and cultured to obtain the virus genome RNA. DNA fragments was acquired by RT-PCR amplification and consistent with the expected HN gene length. The DNA fragment was then cloned into pMD -18 vector and double enzyme digested by coR I and Hind III for verification. The clone was sent Shanghai Sangon Biologica Engineering for sequencing. The sequencing results showed the length of HN gene is 1716bp, encoding 571 amino acids. Homology analysis with 24 published NDV sequences showed the 80% to 98.4% of nucleotide sequence homology and 87.2% to 98.2% of amino acid homology. |
4楼2009-07-27 00:59:00
