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[求助]
关于镍柱重生 已有1人参与
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我想将我做蛋白纯化的镍柱重生，请问方法是什么？ @hc-material @gyesang @gyesang 发自小木虫Android客户端 |
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【答案】应助回帖
感谢参与，应助指数 +1
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步骤如下： Wash column with 3× volume of the resin of Water. Drain completely. Wash column with 3× volume of the resin of 100 mM of EDTA at pH 8.0. Drain completely. Wash column with 5× volume of the resin of Water. Drain completely. Wash column with 1× volume of the resin of 0.2 M NaOH. Drain completely. Wash column with 5× volume of the resin of Water. Drain completely. Wash column with 2× volume of the resin of 0.1 M NiSO4 or NiCl2. Drain completely. Wash column with 5× volume of the resin of Water. Drain completely. Store the resin with 20% ethanol. |
2楼2020-11-26 11:33:54
