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木子木弈

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专业: 生物化学

[求助] 关于镍柱重生已有1人参与

我想将我做蛋白纯化的镍柱重生，请问方法是什么？

@hc-material @gyesang @gyesang 发自小木虫Android客户端

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1楼 2020-11-26 09:20:10

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fuyuandj86

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【答案】应助回帖

感谢参与，应助指数 +1

步骤如下：
Wash column with 3× volume of the resin of Water. Drain completely.
Wash column with 3× volume of the resin of 100 mM of EDTA at pH 8.0. Drain completely.
Wash column with 5× volume of the resin of Water. Drain completely.
Wash column with 1× volume of the resin of 0.2 M NaOH. Drain completely.
Wash column with 5× volume of the resin of Water. Drain completely.
Wash column with 2× volume of the resin of 0.1 M NiSO4 or NiCl2. Drain completely.
Wash column with 5× volume of the resin of Water. Drain completely.
Store the resin with 20% ethanol.

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2楼2020-11-26 11:33:54

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