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²½ÖèÈçÏ£º Wash column with 3¡Á volume of the resin of Water. Drain completely. Wash column with 3¡Á volume of the resin of 100 mM of EDTA at pH 8.0. Drain completely. Wash column with 5¡Á volume of the resin of Water. Drain completely. Wash column with 1¡Á volume of the resin of 0.2 M NaOH. Drain completely. Wash column with 5¡Á volume of the resin of Water. Drain completely. Wash column with 2¡Á volume of the resin of 0.1 M NiSO4 or NiCl2. Drain completely. Wash column with 5¡Á volume of the resin of Water. Drain completely. Store the resin with 20% ethanol. |

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