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北京石油化工学院2026年研究生招生接收调剂公告
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木子木弈

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[求助] 关于镍柱重生 已有1人参与

我想将我做蛋白纯化的镍柱重生,请问方法是什么?

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fuyuandj86

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【答案】应助回帖

感谢参与,应助指数 +1
步骤如下:
    Wash column with 3× volume of the resin of Water. Drain completely.
    Wash column with 3× volume of the resin of 100 mM of EDTA at pH 8.0. Drain completely.
    Wash column with 5× volume of the resin of Water. Drain completely.
    Wash column with 1× volume of the resin of 0.2 M NaOH. Drain completely.
    Wash column with 5× volume of the resin of Water. Drain completely.
    Wash column with 2× volume of the resin of 0.1 M NiSO4 or NiCl2. Drain completely.
    Wash column with 5× volume of the resin of Water. Drain completely.
    Store the resin with 20% ethanol.
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2楼2020-11-26 11:33:54
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