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[求助]
求助各位大神，审稿人提出的这两个问题，我该怎么回应呢
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各位同仁：最近文章送审回来，审稿人提出的两个棘手的问题，请各位帮我考虑考虑我该如何应答，谢谢！ （1）miRNA对应靶标基因验证仅仅做qpcr验证还是不够的，必需要做RACE和降解组测序，原话是：qRT-PCR analysis is not enough,the authors need to validate those tartgets by using RACE or degradome analysis. （2）小RNA测序至少需要做三个重复，你却只做了两个重复.There is a fatal defect in experimental design. The scientific community has established golden standards for small RNA sequencing projects. One of the standards is to have at least three biological replicates for each genotype. The authors only included two biological replicates while claiming differentially expressed miRNAs. This is not acceptable. 请教各位，我该如何作答，谢谢！这篇文章等了半年了，才审稿回来，限定一个月内回复，现在已不可能来补做试验了，请各位大神指点，不胜感激 |
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