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南方科技大学公共卫生及应急管理学院2026级博士研究生招生报考通知(长期有效)
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dongchao

金虫 (小有名气)

[交流] 【请教】怎样证明蛋白的亲和配体

知道某种材料可以特异吸附一种蛋白,怎样证明它们是亲和性吸附,最好再从微观结构的方面证明,请各位指教
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xuguanghai

木虫 (职业作家)

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dongchao(金币+1):谢谢参与
yusen_1982(金币+1):欢迎讨论~ 2010-03-08 14:44
chemist229(金币+1):欢迎讨论交流! 2010-03-09 21:25
Protein A Resin is useful for affinity purification and isolation of IgG. Protein A, a bacterial cell wall protein isolated from Staphylococcus aureus, binds to mammalian IgGs mainly through Fc regions. Native protein A has five IgG binding domains and many other domains of unknown functions. Recombinant protein A contains mainly five high affinity (Ka = 108/M) IgG binding domains with other non-essential domains removed to reduce nonspecific binding. Protein A Resin has been used to isolate and purify classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. Since only the Fc region is involved in binding, the Fab region is available for binding antigens. These qualities of protein A resins combined with GenScript's precision and affordability make GenScript Protein A Resin ideal for the isolation of protein complexes by immunoprecipitation.
4楼2010-03-08 14:22:52
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我是我要飞

金虫 (小有名气)

开开心心

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dongchao(金币+1):谢谢参与
薰衣草儿(金币+1,VIP+0):欢迎讨论~ 11-13 21:20
chemist229(金币+1):欢迎应助! 2010-03-09 21:25
是种什么材料呢?亲和吸附可以看看他们的亲和力常数,用SPR比较好弄
一天到晚不省心
2楼2009-11-13 20:38:08
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mickow

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dongchao(金币+1):谢谢参与
能够通过扩散系数来讨论这个问题吗~?
3楼2010-03-01 22:22:38
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