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dongchao金虫 (小有名气)
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[交流]
【请教】怎样证明蛋白的亲和配体
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| 知道某种材料可以特异吸附一种蛋白,怎样证明它们是亲和性吸附,最好再从微观结构的方面证明,请各位指教 |
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3楼2010-03-01 22:22:38
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2楼2009-11-13 20:38:08
xuguanghai
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dongchao(金币+1):谢谢参与
yusen_1982(金币+1):欢迎讨论~ 2010-03-08 14:44
chemist229(金币+1):欢迎讨论交流! 2010-03-09 21:25
dongchao(金币+1):谢谢参与
yusen_1982(金币+1):欢迎讨论~ 2010-03-08 14:44
chemist229(金币+1):欢迎讨论交流! 2010-03-09 21:25
| Protein A Resin is useful for affinity purification and isolation of IgG. Protein A, a bacterial cell wall protein isolated from Staphylococcus aureus, binds to mammalian IgGs mainly through Fc regions. Native protein A has five IgG binding domains and many other domains of unknown functions. Recombinant protein A contains mainly five high affinity (Ka = 108/M) IgG binding domains with other non-essential domains removed to reduce nonspecific binding. Protein A Resin has been used to isolate and purify classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. Since only the Fc region is involved in binding, the Fab region is available for binding antigens. These qualities of protein A resins combined with GenScript's precision and affordability make GenScript Protein A Resin ideal for the isolation of protein complexes by immunoprecipitation. |
4楼2010-03-08 14:22:52












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