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秦紫蓉

新虫 (初入文坛)

[求助] DNA序列上传NCBI问题求解

在NCBI上传动物COI序列后收到NCBI如下邮件,望有经验前辈赐教,该如何解决。本人比较外行,DNA提取扩增后都是由测序公司测序拼接的,对基因序列中存在的问题也不太清楚。NCBI邮件如下:
We have received the following 57 sequence submissions from you:

SUB7027792 :  (57)

Some or all of the protein coding sequences contain reading frame shifts
(insertions/deletions based on BLAST similarity search results and/or an
alignment), and/or have translations that show little or no similarity
to other proteins in the database.

This generally indicates problems such as:

  - errors in the nucleotide sequence
  - sequences have not been trimmed of low quality
  - problems with feature annotation which may be due to:
        translation in the wrong reading frame or on the wrong strand
        translation does not have the correct exon spans
        translation includes noncoding sequence, such as introns or UTR's

Provide a corrected submission file through the Submission Portal:
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尛哲仙聖

新虫 (初入文坛)

我也出现了这种情况 不知道该如何处理了
5楼2023-07-12 17:53:48
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年少轻狂xf

新虫 (正式写手)

2楼2020-04-07 00:51:58
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weebug

版主 (著名写手)

序列上有增减,导致转译到蛋白氨基酸时发生错误。
天蓝蓝兮日头晒
3楼2020-04-07 09:58:11
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KelvinLeo

新虫 (小有名气)

可以参考文库教程搜索"编码蛋白质的基因序列提交”

发自小木虫Android客户端
4楼2021-03-24 15:09:36
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