24小时热门版块排行榜    

查看: 1358  |  回复: 4

秦紫蓉

新虫 (初入文坛)

[求助] DNA序列上传NCBI问题求解

在NCBI上传动物COI序列后收到NCBI如下邮件,望有经验前辈赐教,该如何解决。本人比较外行,DNA提取扩增后都是由测序公司测序拼接的,对基因序列中存在的问题也不太清楚。NCBI邮件如下:
We have received the following 57 sequence submissions from you:

SUB7027792 :  (57)

Some or all of the protein coding sequences contain reading frame shifts
(insertions/deletions based on BLAST similarity search results and/or an
alignment), and/or have translations that show little or no similarity
to other proteins in the database.

This generally indicates problems such as:

  - errors in the nucleotide sequence
  - sequences have not been trimmed of low quality
  - problems with feature annotation which may be due to:
        translation in the wrong reading frame or on the wrong strand
        translation does not have the correct exon spans
        translation includes noncoding sequence, such as introns or UTR's

Provide a corrected submission file through the Submission Portal:
回复此楼
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖

年少轻狂xf

新虫 (正式写手)

2楼2020-04-07 00:51:58
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖

weebug

版主 (著名写手)

序列上有增减,导致转译到蛋白氨基酸时发生错误。
天蓝蓝兮日头晒
3楼2020-04-07 09:58:11
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖

KelvinLeo

新虫 (小有名气)

可以参考文库教程搜索"编码蛋白质的基因序列提交”

发自小木虫Android客户端
4楼2021-03-24 15:09:36
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖

尛哲仙聖

新虫 (初入文坛)

我也出现了这种情况 不知道该如何处理了
5楼2023-07-12 17:53:48
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖
相关版块跳转 我要订阅楼主 秦紫蓉 的主题更新
最具人气热帖推荐 [查看全部] 作者 回/看 最后发表
[考研] 0856材料专硕353求调剂 +4 NIFFFfff 2026-03-20 4/200 2026-03-22 09:49 by 2026paper
[考研] 求调剂 +4 要好好无聊 2026-03-21 4/200 2026-03-21 18:57 by 学员8dgXkO
[考研] 313求调剂 +4 肆叁贰壹22 2026-03-19 4/200 2026-03-21 17:33 by ColorlessPI
[考研] 307求调剂 +3 余意卿 2026-03-18 3/150 2026-03-21 17:31 by ColorlessPI
[考研] 材料 271求调剂 +5 展信悦_ 2026-03-21 5/250 2026-03-21 17:29 by 学员8dgXkO
[考研] 296求调剂 +4 www_q 2026-03-20 4/200 2026-03-21 17:26 by 学员8dgXkO
[考研] 266求调剂 +3 哇呼哼呼哼 2026-03-20 3/150 2026-03-21 16:46 by barlinike
[考研] 一志愿重庆大学085700资源与环境总分308求调剂 +7 墨墨漠 2026-03-20 7/350 2026-03-21 16:36 by barlinike
[考研] 070300化学319求调剂 +7 锦鲤0909 2026-03-17 7/350 2026-03-21 03:46 by JourneyLucky
[考研] 一志愿华中科技大学,080502,354分求调剂 +5 守候夕阳CF 2026-03-18 5/250 2026-03-21 01:06 by JourneyLucky
[考研] 274求调剂 +10 S.H1 2026-03-18 10/500 2026-03-20 23:51 by JourneyLucky
[考研] 287求调剂 +7 晨昏线与星海 2026-03-19 8/400 2026-03-20 22:19 by JourneyLucky
[考研] 085600材料与化工 +8 安全上岸! 2026-03-16 8/400 2026-03-20 22:13 by luoyongfeng
[考研] 求调剂 +3 eation27 2026-03-20 3/150 2026-03-20 19:32 by JourneyLucky
[考研] 工科材料085601 279求调剂 +7 困于星晨 2026-03-17 9/450 2026-03-20 17:38 by 无懈可击111
[论文投稿] 申请回稿延期一个月,编辑同意了。但系统上的时间没变,给编辑又写邮件了,没回复 10+3 wangf9518 2026-03-17 4/200 2026-03-19 23:55 by babero
[考研] 312求调剂 +8 陌宸希 2026-03-16 9/450 2026-03-18 12:39 by Linda Hu
[考研] 材料,纺织,生物(0856、0710),化学招生啦 +3 Eember. 2026-03-17 9/450 2026-03-18 10:28 by Eember.
[考研] 277调剂 +5 自由煎饼果子 2026-03-16 6/300 2026-03-17 19:26 by 李leezz
[考研] 一志愿,福州大学材料专硕339分求调剂 +3 木子momo青争 2026-03-15 3/150 2026-03-17 07:52 by laoshidan
信息提示
请填处理意见