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【答案】应助回帖
★ ★ ★ ★ ★ ★ ★ ★ ★ ★
感谢参与，应助指数 +1
似水含笑(心静_依然代发): 金币+10, 感谢应助 2019-06-11 08:49:58
心静_依然: LS-EPI+1, 感谢应助 2019-06-11 08:50:04
sci已经可以检索到
第 1 条，共 1 条
标题: Bacteria-immobilized Prepartion as the Microbial Probe for Electrochemical Susceptibility Test
作者: Han, X (Han, Xiao); Chen, LZ (Chen, Lizhi); He, D (He, Di); He, JT (He, Jingting); Ma, Y (Ma, Ye); Wang, JL (Wang, Jingling); Liu, C (Liu, Chang)
来源出版物: INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE 卷: 14 期: 3 页: 2833-2845 DOI: 10.20964/2019.03.45 出版年: MAR 2019
摘要: Electrochemical measurement of respiratory activity based on a multilayered composite of bacteria has been developed for susceptibility test. Here, we firstly report the successful fabrication of graphene oxide (GO)/polyvinyl alcohol (PVA)/Escherichia coli (E. coli)/GO/graphite rod (GR) composite, where the GO/GR composite was prepared by chemical oxidation and then the E. coli was confined at the surface of GO/GR composite by in site culture and crosslink of GO/PVA in sequence. Although it induced 29.07% decrease in the sensitivity of immobilized E. coli to toxic chemicals, the presence of GO/PVA could promote the storage stability for 20 days in term of relative bioactivity. The GO/PVA/E. coli/GO/GR composite was used as the bacterial probe in the electrochemical sensor for susceptibility test of antibiotics, and the IC50 values were determined to be 8.01 mg/L, 6.51 mg/L, 9.93 mg/L and 4.07 mg/L for gentamicin, amikacin, amoxicillin and cefixime, which were identical with those obtained with standard Kirby-Bauer disk susceptibility (KB) testing. The vancomycin, only affecting gram-positive bacteria, exhibited an inhibition ratio of 34.5% at the concentration of 16.0 mg/L in the electrochemical susceptibility test, agreeing with the result from KB testing.
入藏号: WOS:000460758200055
ISSN: 1452-3981 |
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