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ÇóÖú¡ªÄÄλ´óÉñÄÜÔÚpubmed»òÕßsciÍøÕ¾ÉϼìË÷³öÏÂÃæÎÄÏ×£ºBacteria-immobilized Prepartion as the Microbial Probe for Electrochemical Susceptibility Test
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ËÆË®º¬Ð¦(Ðľ²_ÒÀÈ»´ú·¢): ½ð±Ò+10, ¸ÐлӦÖú 2019-06-11 08:49:58
Ðľ²_ÒÀÈ»: LS-EPI+1, ¸ÐлӦÖú 2019-06-11 08:50:04
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±êÌâ: Bacteria-immobilized Prepartion as the Microbial Probe for Electrochemical Susceptibility Test
×÷Õß: Han, X (Han, Xiao); Chen, LZ (Chen, Lizhi); He, D (He, Di); He, JT (He, Jingting); Ma, Y (Ma, Ye); Wang, JL (Wang, Jingling); Liu, C (Liu, Chang)
À´Ô´³ö°æÎï: INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE  ¾í: 14  ÆÚ: 3  Ò³: 2833-2845  DOI: 10.20964/2019.03.45  ³ö°æÄê: MAR 2019  
ÕªÒª: Electrochemical measurement of respiratory activity based on a multilayered composite of bacteria has been developed for susceptibility test. Here, we firstly report the successful fabrication of graphene oxide (GO)/polyvinyl alcohol (PVA)/Escherichia coli (E. coli)/GO/graphite rod (GR) composite, where the GO/GR composite was prepared by chemical oxidation and then the E. coli was confined at the surface of GO/GR composite by in site culture and crosslink of GO/PVA in sequence. Although it induced 29.07% decrease in the sensitivity of immobilized E. coli to toxic chemicals, the presence of GO/PVA could promote the storage stability for 20 days in term of relative bioactivity. The GO/PVA/E. coli/GO/GR composite was used as the bacterial probe in the electrochemical sensor for susceptibility test of antibiotics, and the IC50 values were determined to be 8.01 mg/L, 6.51 mg/L, 9.93 mg/L and 4.07 mg/L for gentamicin, amikacin, amoxicillin and cefixime, which were identical with those obtained with standard Kirby-Bauer disk susceptibility (KB) testing. The vancomycin, only affecting gram-positive bacteria, exhibited an inhibition ratio of 34.5% at the concentration of 16.0 mg/L in the electrochemical susceptibility test, agreeing with the result from KB testing.
Èë²ØºÅ: WOS:000460758200055
ISSN: 1452-3981
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