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hulqi

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专业: 微生物资源与分类学

[交流] 关于蛋白结构预测和信号肽的问题

我有一个已知序列的基因。使用TMHMM预测有8次穿膜：
# Sequence Length: 364
# Sequence Number of predicted TMHs:  8
# Sequence Exp number of AAs in TMHs: 169.90887
# Sequence Exp number, first 60 AAs:  42.41761
# Sequence Total prob of N-in:       0.01895
# Sequence POSSIBLE N-term signal sequence
Sequence TMHMM2.0 outside       1    3
Sequence TMHMM2.0 TMhelix       4 26
Sequence TMHMM2.0 inside       27 38
Sequence TMHMM2.0 TMhelix       39 61
Sequence TMHMM2.0 outside       62 91
Sequence TMHMM2.0 TMhelix       92 111
Sequence TMHMM2.0 inside    112 117
Sequence TMHMM2.0 TMhelix    118 137
Sequence TMHMM2.0 outside    138 156
Sequence TMHMM2.0 TMhelix    157 179
Sequence TMHMM2.0 inside    180 191
Sequence TMHMM2.0 TMhelix    192 214
Sequence TMHMM2.0 outside    215 228
Sequence TMHMM2.0 TMhelix    229 251
Sequence TMHMM2.0 inside    252 300
Sequence TMHMM2.0 TMhelix    301 323
Sequence TMHMM2.0 outside    324 364

但是用SignalP 3.0 Server 检测信号肽的强度时得出这样的结果：
>Sequence             length = 70
# Measure  Position  Value  Cutoff  signal peptide?
  max. C 18    0.159 0.52 NO
  max. Y 18    0.088 0.33 NO
  max. S 10    0.643 0.92 NO
  mean S    1-17 0.228 0.49 NO
      D    1-17 0.158 0.44 NO

>Sequence
Prediction: Non-secretory protein
Signal peptide probability: 0.048
Max cleavage site probability: 0.021 between pos. 27 and 28

两者结论看上去是矛盾的啊。这么多次穿膜信号肽应该很强才对啊。哪位大侠帮我解释一下，多谢了~~

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