24小时热门版块排行榜

返回列表

本帖产生 1 个，点击这里进行查看

xiandongzeng

木虫 (正式写手)

应助: 0 (幼儿园)
金币: 2170.7
散金: 6
帖子: 316
在线: 252小时
虫号: 400963
注册: 2007-06-13
性别: GG
专业: 色谱分析

[求助] WOS入藏号求助

WOS入藏号求助：Next-generation sequencing reveals lymph node metastasis associated genetic markers in colorectal cancer，Experimental & Therapeutic Medicine , 2017 , 14 (1) :338。

回复此楼

» 猜你喜欢

1楼 2018-09-17 17:04:54

已阅回复此楼关注TA 给TA发消息送TA红花 TA的回帖

baiyuefei

版主 (文学泰斗)

风雪

应助: 4643 (副教授)
贵宾: 46.97
金币: 659003
散金: 11616
红花: 995
沙发: 81
帖子: 69548
在线: 13456.9小时
虫号: 676696
注册: 2008-12-18
性别: GG
专业: 合成药物化学
管辖: 有机交流

【答案】应助回帖

★ ★ ★ ★ ★
感谢参与，应助指数 +1
xiandongzeng: 金币+5, ★★★★★最佳答案, 3Ks 2018-09-18 09:15:12
心静_依然: LS-EPI+1, 感谢应助 2018-09-19 08:38:11

Next-generation sequencing reveals lymph node metastasis associated genetic markers in colorectal cancer
作者:Xie, N (Xie, Ni)[ 1 ] ; Yao, YJ (Yao, Yujiang)[ 1 ] ; Wan, LL (Wan, Lili)[ 1 ] ; Zhu, T (Zhu, Ting)[ 1 ] ; Liu, LT (Liu, Litao)[ 1 ] ; Yuan, JH (Yuan, Jianhui)[ 2 ]

EXPERIMENTAL AND THERAPEUTIC MEDICINE

卷: 14  期: 1  页: 338-343  子辑: A
DOI: 10.3892/etm.2017.4464

出版年:JUL 2017

文献类型:Article

查看期刊影响力

摘要
Colorectal cancer is the third most prevalent type of cancer in the United States. Early diagnosis of lymph node metastases is essential to improve the prognosis for patients with colorectal cancer. Therefore, the present study aimed to screen genetic markers, including single nucleotide polymorphisms (SNPs), copy number variations (CNVs) and mRNA expression, associated with lymph node metastases in patients with colorectal cancer to enable an early diagnosis. Targeted next-generation sequencing was applied to capture SNPs and CNVs in tumor-related candidate genes within tumor tissues from 39 colorectal cancer patients; reverse transcription-quantitative polymerase chain reaction was used to detect the specific mRNA level of tumor-related candidate genes, including vascular endothelial growth factor C, cyclin-A2, Interleukin-2, ATP-binding cassette sub-family G member 2, epidermal growth factor (EGF) and nuclear factor kappa B subunit 1 (NFKB1) on chromosome 4. The SNPs in solute carrier family 28 member 3 (SLC28A3), breast cancer 1 (BRCA1), ribonucleotide reductase regulators subunit M2 (RRM2), PMS1 homolog 2 (PMS2), cytidine deaminase (CDA), epoxide hydrolase 1 (EPHX1), heterogenous ribonucleoprotein particle-associated with lethal yellow (RALY), Siglec-3 (CD33), B cell lymphoma 10 (BCL10), ETS variant 1 (ETV1), macrophage stimulating 1 receptor 1 (MST1R), lysine methyltransferase 2B (KMT2B), B cell lymphoma 2 (BCL2), U6 small nuclear RNA -associated Sm-like protein 3 (LSM3), thyroid transcription factor 1 (TTF1) and mitogen-activated protein 3 kinase 1 (MAP3K1) were significantly associated with lymphatic metastasis (P<0.05). EGF and NFKB1 were both observed to be significantly downregulated in the lymph node metastases group (P<0.05). Although no association between CNVs and lymph node metastases in patients with colorectal cancer was observed in the present study, SNPs in SLC28A3, BRCA1, RRM2, PMS2, CDA, EPHX1, RALY, CD33, BCL10, ETV1, MST1R, KMT2B, BCL2, LSM3, TTF1 and MAP3K1 were significantly associated with colorectal cancer. Downregulation of EGF and NFKB1 was also identified to be associated with lymph node metastases in colorectal cancer. The findings of the current study provide a scientific basis for the clinical inspection of lymphatic metastasis and prognosis prediction, intervention and guidance therapy for patients with colorectal cancer.

关键词
作者关键词:copy number variation; single nucleotide polymorphisms; colorectal cancer; lymph node metastasis

KeyWords Plus:COMPARATIVE GENOMIC HYBRIDIZATION; RECTAL-CANCER; OPPORTUNITIES; PATHOGENESIS; TRANSITION; PATHWAYS

作者信息
通讯作者地址: Yuan, JH (通讯作者)

      Shenzhen Ctr Dis Control & Prevent, Sci & Educ Adm Dept, 8 Longyuan Rd, Shenzhen 518055, Guangdong, Peoples R China.
地址:

显示更多 [ 1 ] Shenzhen Second Peoples Hosp, Sci & Educ Adm Dept, Shenzhen 518000, Guangdong, Peoples R China
      [ 2 ] Shenzhen Ctr Dis Control & Prevent, Sci & Educ Adm Dept, 8 Longyuan Rd, Shenzhen 518055, Guangdong, Peoples R China
电子邮件地址:jianhui_yuan@126.com

基金资助致谢
基金资助机构授权号
Project of Basic Research Plan Program of Shenzhen
JCYJ20160328161613864
Innovation Program of Shenzhen
JCYJ20150330102720122
Project of Shenzhen Basic Development Program
JCYJ20120613171430264
JCYJ20130329110928684
Project of Shenzhen International Cooperation Foundation
GJHZ20130412153906740
GJHZ20160301163138685
Nature Science Foundation of Guangdong
2016A030313029
Project of Guangdong Scientific Plan Foundation
2013B021800097
2014A020212038
查看基金资助信息
出版商
SPANDIDOS PUBL LTD, POB 18179, ATHENS, 116 10, GREECE

类别 / 分类
研究方向:Research & Experimental Medicine

Web of Science 类别:Medicine, Research & Experimental

文献信息
语言:English

入藏号: WOS:000405645400050

PubMed ID: 28672935

ISSN: 1792-0981

eISSN: 1792-1015

其他信息
IDS 号: FA7RU

Web of Science 核心合集中的 "引用的参考文献": 26

Web of Science 核心合集中的 "被引频次": 2

回复此楼

2楼2018-09-18 08:46:31

已阅回复此楼关注TA 给TA发消息送TA红花 TA的回帖

相关版块跳转我要订阅楼主 xiandongzeng 的主题更新

返回列表