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【答案】应助回帖
★ ★ ★ ★ ★
感谢参与，应助指数 +1
xiandongzeng: 金币+5, ★★★★★最佳答案, 3Ks 2018-09-18 09:15:12
心静_依然: LS-EPI+1, 感谢应助 2018-09-19 08:38:11
Next-generation sequencing reveals lymph node metastasis associated genetic markers in colorectal cancer
作者:Xie, N (Xie, Ni)[ 1 ] ; Yao, YJ (Yao, Yujiang)[ 1 ] ; Wan, LL (Wan, Lili)[ 1 ] ; Zhu, T (Zhu, Ting)[ 1 ] ; Liu, LT (Liu, Litao)[ 1 ] ; Yuan, JH (Yuan, Jianhui)[ 2 ]
EXPERIMENTAL AND THERAPEUTIC MEDICINE
卷: 14 期: 1 页: 338-343 子辑: A
DOI: 10.3892/etm.2017.4464
出版年:JUL 2017
文献类型:Article
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摘要
Colorectal cancer is the third most prevalent type of cancer in the United States. Early diagnosis of lymph node metastases is essential to improve the prognosis for patients with colorectal cancer. Therefore, the present study aimed to screen genetic markers, including single nucleotide polymorphisms (SNPs), copy number variations (CNVs) and mRNA expression, associated with lymph node metastases in patients with colorectal cancer to enable an early diagnosis. Targeted next-generation sequencing was applied to capture SNPs and CNVs in tumor-related candidate genes within tumor tissues from 39 colorectal cancer patients; reverse transcription-quantitative polymerase chain reaction was used to detect the specific mRNA level of tumor-related candidate genes, including vascular endothelial growth factor C, cyclin-A2, Interleukin-2, ATP-binding cassette sub-family G member 2, epidermal growth factor (EGF) and nuclear factor kappa B subunit 1 (NFKB1) on chromosome 4. The SNPs in solute carrier family 28 member 3 (SLC28A3), breast cancer 1 (BRCA1), ribonucleotide reductase regulators subunit M2 (RRM2), PMS1 homolog 2 (PMS2), cytidine deaminase (CDA), epoxide hydrolase 1 (EPHX1), heterogenous ribonucleoprotein particle-associated with lethal yellow (RALY), Siglec-3 (CD33), B cell lymphoma 10 (BCL10), ETS variant 1 (ETV1), macrophage stimulating 1 receptor 1 (MST1R), lysine methyltransferase 2B (KMT2B), B cell lymphoma 2 (BCL2), U6 small nuclear RNA -associated Sm-like protein 3 (LSM3), thyroid transcription factor 1 (TTF1) and mitogen-activated protein 3 kinase 1 (MAP3K1) were significantly associated with lymphatic metastasis (P<0.05). EGF and NFKB1 were both observed to be significantly downregulated in the lymph node metastases group (P<0.05). Although no association between CNVs and lymph node metastases in patients with colorectal cancer was observed in the present study, SNPs in SLC28A3, BRCA1, RRM2, PMS2, CDA, EPHX1, RALY, CD33, BCL10, ETV1, MST1R, KMT2B, BCL2, LSM3, TTF1 and MAP3K1 were significantly associated with colorectal cancer. Downregulation of EGF and NFKB1 was also identified to be associated with lymph node metastases in colorectal cancer. The findings of the current study provide a scientific basis for the clinical inspection of lymphatic metastasis and prognosis prediction, intervention and guidance therapy for patients with colorectal cancer.
关键词
作者关键词:copy number variation; single nucleotide polymorphisms; colorectal cancer; lymph node metastasis
KeyWords Plus:COMPARATIVE GENOMIC HYBRIDIZATION; RECTAL-CANCER; OPPORTUNITIES; PATHOGENESIS; TRANSITION; PATHWAYS
作者信息
通讯作者地址: Yuan, JH (通讯作者)
Shenzhen Ctr Dis Control & Prevent, Sci & Educ Adm Dept, 8 Longyuan Rd, Shenzhen 518055, Guangdong, Peoples R China.
地址:
显示更多 [ 1 ] Shenzhen Second Peoples Hosp, Sci & Educ Adm Dept, Shenzhen 518000, Guangdong, Peoples R China
[ 2 ] Shenzhen Ctr Dis Control & Prevent, Sci & Educ Adm Dept, 8 Longyuan Rd, Shenzhen 518055, Guangdong, Peoples R China
电子邮件地址:jianhui_yuan@126.com
基金资助致谢
基金资助机构 授权号
Project of Basic Research Plan Program of Shenzhen
JCYJ20160328161613864
Innovation Program of Shenzhen
JCYJ20150330102720122
Project of Shenzhen Basic Development Program
JCYJ20120613171430264
JCYJ20130329110928684
Project of Shenzhen International Cooperation Foundation
GJHZ20130412153906740
GJHZ20160301163138685
Nature Science Foundation of Guangdong
2016A030313029
Project of Guangdong Scientific Plan Foundation
2013B021800097
2014A020212038
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出版商
SPANDIDOS PUBL LTD, POB 18179, ATHENS, 116 10, GREECE
类别 / 分类
研究方向:Research & Experimental Medicine
Web of Science 类别:Medicine, Research & Experimental
文献信息
语言:English
入藏号: WOS:000405645400050
PubMed ID: 28672935
ISSN: 1792-0981
eISSN: 1792-1015
其他信息
IDS 号: FA7RU
Web of Science 核心合集中的 "引用的参考文献": 26
Web of Science 核心合集中的 "被引频次": 2 |
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