24小时热门版块排行榜    

Znn3bq.jpeg
查看: 2838  |  回复: 5
当前主题已经存档。

bxf820726

[交流] 【求助】急求BSA的分子量是多少?

各位大虾:
         BSA的分子量是多少呀?
回复此楼
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖

aegeansyang

银虫 (正式写手)


bxf820726(金币+1):谢谢参与
~67000Da
2楼2009-04-02 10:12:22
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖

yurui0211

金虫 (正式写手)


yusen_1982(金币+1,VIP+0):欢迎应助 4-2 13:43
An Homogeneous Protein

    Bovine serum albumin (BSA) is one of the most commonly used carriers for conjugation in antibody production. It belongs to the class of serum proteins called albumins, which make up about half of the protein in plasma and are the most stable and soluble proteins in plasma. It is very popular with laboratories developing immunoassays, mostly due to its availability, solubility and the numerous functional groups present for coupling to heptans.

Molecular Weight

    Molecular weight (MW): The MW of BSA is 67 000 which is much smaller than that of KLH, another commonly used carrier protein. It is, however, much more soluble and is immunogenic. It contains 59 lysines, and 30-35 of these are primary amines capable of reacting with a conjugation sites of linkers. It is a popular carrier for weakly antigenic compounds.

Solubility

    Solubility in aqueous solution: BSA is easily dissolved in an aqueous solution. It can be highly purified to become homogeneous. BSA is commonly used as protein standard in protein assays and as a molecular weight standard for SDS-PAGE and gel permeation chromatography.

Applications

    BSA can be used to block nonspecific binding sites in many immunochemical experiments such as ELISA, immunoblotting and immunohistochemical studies. It may be used as a non-relevant protein in enzyme immunoassays; KLH cannot be used for this because the anti-KLH antibodies, which formed during immunization, will interfere with the measurement of anti-heptan antibodies. When KLH is used as the carrier, heptan-BSA conjugates can be used because they do not interfere with the measurement for anti-heptan antibodies.
临渊羡鱼,不如退而结网!
3楼2009-04-02 13:09:11
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖

无知3096

木虫 (小有名气)

一起前进吧!
4楼2009-04-02 13:57:42
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖

wangbaobao602

金虫 (小有名气)


yusen_1982(金币+1,VIP+0):欢迎应助! 4-3 21:23
分子量68kD。等电点4.8。
5楼2009-04-03 16:59:18
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖

funnyduck

金虫 (小有名气)


yusen_1982(金币+1,VIP+0):欢迎应助 4-23 09:01
MV=66kDa, pI=4.7
6楼2009-04-23 03:00:32
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖
相关版块跳转 我要订阅楼主 bxf820726 的主题更新
普通表情 高级回复 (可上传附件)
最具人气热帖推荐 [查看全部] 作者 回/看 最后发表
[考研] 085801电气专硕272求调剂 +19 电气李 2026-04-13 21/1050 2026-04-15 13:37 by 黑科技矿业
[考研] 求助调剂,跨调 +18 X十甫寸Y 2026-04-11 19/950 2026-04-14 19:26 by Art1977
[考研] 材料299专硕求调剂 +16 +21 2026-04-09 16/800 2026-04-14 17:06 by jiangguiquan11
[考研] 药学305求调剂 +10 玛卡巴卡boom 2026-04-10 10/500 2026-04-14 15:55 by zs92450
[考研] 求调剂 +12 何气正 2026-04-13 13/650 2026-04-14 14:47 by zs92450
[考研] 300分求调剂 (085501机械专硕,本科扬大) +9 xu@841019 2026-04-11 10/500 2026-04-14 08:48 by 木木mumu~
[考研] 考研求调剂 +12 子木呐 2026-04-12 13/650 2026-04-14 01:19 by 王珺璞
[考研] 一志愿211 0703化学 346分求调剂 +26 土豆er? 2026-04-09 29/1450 2026-04-13 15:15 by 独醉梦孤城
[考研] 一志愿浙大生物325分求调剂 +9 zysheng 2026-04-12 9/450 2026-04-12 22:31 by yuyin1233
[考研] 295分求调剂 +13 ?要上岸? 2026-04-10 13/650 2026-04-12 15:37 by laoshidan
[考研] 材料工程日语考生求调剂 +7 0856?调剂 2026-04-10 7/350 2026-04-11 21:33 by 蓝云思雨
[考研] 电子信息279求调剂,有书读就行 +8 wwwooden 2026-04-08 11/550 2026-04-11 20:22 by cq2548
[考研] 085600材料与化工329分求调剂 +16 叶zilin 2026-04-10 16/800 2026-04-11 11:04 by may_新宇
[考研] 工科273调剂 +6 X1999 2026-04-09 7/350 2026-04-11 10:23 by zhq0425
[考研] 289 分105500药学专硕求调剂(找B区学校) +6 白云123456789 2026-04-09 8/400 2026-04-10 21:13 by zhouxiaoyu
[考研] 一志愿华东师范生物学326分,求调剂 +8 刘墨墨 2026-04-09 8/400 2026-04-10 12:00 by pengliang8036
[考研] 本科211 工科085400 280分求调剂 可跨专业 +3 LZH(等待调剂中 2026-04-09 3/150 2026-04-09 21:29 by wutongshun
[考研] 调剂 +19 2261744733 2026-04-08 19/950 2026-04-09 19:11 by vgtyfty
[考研] 085501机械英二77总分294求调剂,接受跨专业学习 +6 守法公民亓纪 2026-04-08 6/300 2026-04-09 15:55 by wp06
[考研] 求调剂 +3 猪肉墩粉条cc 2026-04-08 4/200 2026-04-09 10:05 by 猪肉墩粉条cc
信息提示
请填处理意见