| 查看: 2838 | 回复: 5 | |||
| 当前主题已经存档。 | |||
[交流]
【求助】急求BSA的分子量是多少？
|
|||
|
各位大虾: BSA的分子量是多少呀? |
回复此楼» 猜你喜欢
08开头
已经有0人回复
-
中科中山药物创新研究院（药创院）怎么样？
已经有0人回复
-
生物物理、生物化学与分子生物学论文润色/翻译怎么收费?
已经有116人回复
» 本主题相关商家推荐: (我也要在这里推广)
aegeansyang
银虫 (正式写手)
- 应助: 12 (小学生)
- 金币: 5517.1
- 散金: 1825
- 红花: 5
- 帖子: 856
- 在线: 724.4小时
- 虫号: 243919
- 注册: 2006-04-16
- 性别: GG
- 专业: 组织工程学
2楼2009-04-02 10:12:22
★
yusen_1982(金币+1,VIP+0):欢迎应助 4-2 13:43
yusen_1982(金币+1,VIP+0):欢迎应助 4-2 13:43
|
An Homogeneous Protein Bovine serum albumin (BSA) is one of the most commonly used carriers for conjugation in antibody production. It belongs to the class of serum proteins called albumins, which make up about half of the protein in plasma and are the most stable and soluble proteins in plasma. It is very popular with laboratories developing immunoassays, mostly due to its availability, solubility and the numerous functional groups present for coupling to heptans. Molecular Weight Molecular weight (MW): The MW of BSA is 67 000 which is much smaller than that of KLH, another commonly used carrier protein. It is, however, much more soluble and is immunogenic. It contains 59 lysines, and 30-35 of these are primary amines capable of reacting with a conjugation sites of linkers. It is a popular carrier for weakly antigenic compounds. Solubility Solubility in aqueous solution: BSA is easily dissolved in an aqueous solution. It can be highly purified to become homogeneous. BSA is commonly used as protein standard in protein assays and as a molecular weight standard for SDS-PAGE and gel permeation chromatography. Applications BSA can be used to block nonspecific binding sites in many immunochemical experiments such as ELISA, immunoblotting and immunohistochemical studies. It may be used as a non-relevant protein in enzyme immunoassays; KLH cannot be used for this because the anti-KLH antibodies, which formed during immunization, will interfere with the measurement of anti-heptan antibodies. When KLH is used as the carrier, heptan-BSA conjugates can be used because they do not interfere with the measurement for anti-heptan antibodies. |
3楼2009-04-02 13:09:11
无知3096
木虫 (小有名气)
- 应助: 0 (幼儿园)
- 金币: 2343.4
- 红花: 1
- 帖子: 141
- 在线: 413.8小时
- 虫号: 589938
- 注册: 2008-08-30
- 性别: GG
- 专业: 环境科学 nano
4楼2009-04-02 13:57:42
wangbaobao602
金虫 (小有名气)
- 应助: 0 (幼儿园)
- 金币: 1192
- 散金: 2
- 帖子: 130
- 在线: 54.9小时
- 虫号: 489279
- 注册: 2008-01-02
- 性别: GG
- 专业: 分离过程
5楼2009-04-03 16:59:18
funnyduck
金虫 (小有名气)
- 应助: 2 (幼儿园)
- 金币: 1864.7
- 红花: 3
- 帖子: 76
- 在线: 135.1小时
- 虫号: 708891
- 注册: 2009-02-26
- 性别: GG
- 专业: 分离过程
6楼2009-04-23 03:00:32
