| 查看: 3122 | 回复: 5 | |||
| 当前主题已经存档。 | |||
[交流]
【求助】急求BSA的分子量是多少？
|
|||
|
各位大虾: BSA的分子量是多少呀? |
回复此楼» 猜你喜欢
赋能青年学术发声，携手打造前沿特刊，Biofunctional Materials 青年编委全球招募
已经有7人回复
-
院士领衔，ICBMBA生物功能材料与生物医学应用会议诚邀学者加入！
已经有6人回复
-
生物物理、生物化学与分子生物学论文润色/翻译怎么收费?
已经有144人回复
-
ICBMBA 2026，生物功能材料与生物医学应用国际会议诚邀参与！
已经有5人回复
-
Interdiscipline 2026，2026国际交叉学大会，多学科诚邀参会，院士领衔，专家云集
已经有5人回复
-
【PhD 招生 | La Trobe University | 有机合成 / 化学生物学 / 多肽化学】
已经有24人回复
-
聊聊生物材料课题从合成到验证的那些事
已经有0人回复
-
【科普】医工交叉漫谈：水凝胶凭什么成为组织修复热门材料？
已经有0人回复
-
重磅邀约｜2026 生物医药产业发展大会
已经有0人回复
-
关于叠氮和炔的CuAAC工艺问题
已经有0人回复
-
多赖氨酸的选择性修饰
已经有0人回复
» 本主题相关商家推荐: (我也要在这里推广)
aegeansyang
银虫 (正式写手)
- 应助: 12 (小学生)
- 金币: 5517.1
- 散金: 1825
- 红花: 5
- 帖子: 856
- 在线: 724.4小时
- 虫号: 243919
- 注册: 2006-04-16
- 性别: GG
- 专业: 组织工程学
2楼2009-04-02 10:12:22
★
yusen_1982(金币+1,VIP+0):欢迎应助 4-2 13:43
yusen_1982(金币+1,VIP+0):欢迎应助 4-2 13:43
|
An Homogeneous Protein Bovine serum albumin (BSA) is one of the most commonly used carriers for conjugation in antibody production. It belongs to the class of serum proteins called albumins, which make up about half of the protein in plasma and are the most stable and soluble proteins in plasma. It is very popular with laboratories developing immunoassays, mostly due to its availability, solubility and the numerous functional groups present for coupling to heptans. Molecular Weight Molecular weight (MW): The MW of BSA is 67 000 which is much smaller than that of KLH, another commonly used carrier protein. It is, however, much more soluble and is immunogenic. It contains 59 lysines, and 30-35 of these are primary amines capable of reacting with a conjugation sites of linkers. It is a popular carrier for weakly antigenic compounds. Solubility Solubility in aqueous solution: BSA is easily dissolved in an aqueous solution. It can be highly purified to become homogeneous. BSA is commonly used as protein standard in protein assays and as a molecular weight standard for SDS-PAGE and gel permeation chromatography. Applications BSA can be used to block nonspecific binding sites in many immunochemical experiments such as ELISA, immunoblotting and immunohistochemical studies. It may be used as a non-relevant protein in enzyme immunoassays; KLH cannot be used for this because the anti-KLH antibodies, which formed during immunization, will interfere with the measurement of anti-heptan antibodies. When KLH is used as the carrier, heptan-BSA conjugates can be used because they do not interfere with the measurement for anti-heptan antibodies. |
3楼2009-04-02 13:09:11
无知3096
木虫 (小有名气)
- 应助: 0 (幼儿园)
- 金币: 2343.4
- 红花: 1
- 帖子: 141
- 在线: 413.8小时
- 虫号: 589938
- 注册: 2008-08-30
- 性别: GG
- 专业: 环境科学 nano
4楼2009-04-02 13:57:42
wangbaobao602
金虫 (小有名气)
- 应助: 0 (幼儿园)
- 金币: 1192
- 散金: 2
- 帖子: 130
- 在线: 54.9小时
- 虫号: 489279
- 注册: 2008-01-02
- 性别: GG
- 专业: 分离过程
5楼2009-04-03 16:59:18
funnyduck
金虫 (小有名气)
- 应助: 2 (幼儿园)
- 金币: 1864.7
- 红花: 3
- 帖子: 76
- 在线: 135.1小时
- 虫号: 708891
- 注册: 2009-02-26
- 性别: GG
- 专业: 分离过程
6楼2009-04-23 03:00:32
