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[求助] 壳聚糖-戊二醛-希夫碱反应用量

大家好，我参照文献做壳聚糖和戊二醛的希夫碱反应，文献中的描述如下：
   CHIT was modified with GDI by adding dropwise the 0.10 wt % CHIT solution to a 50 wt % GDI solution until the molar ratio of GDI to chitosan glucosamine units was 200:1. The mixture was stirred at room temperature for 24 h, and the unreacted GDI was separated from the CHIT-GDI product by multiple extractions with ethyl ether.
   请问“until the molar ratio of GDI to chitosan glucosamine units was 200:1”，要达到这里要求的200:1，50 wt%的戊二醛和0.1 wt%各需要多少体积？使用的壳聚糖是sigma买的：mol wt 为 mol wt 50,000-190,000 Da (based on viscosity)，Physical form 75-85% 脱乙酰化。
   或者能告诉我怎么计算壳聚糖（mol wt 为 mol wt 50,000-190,000 Da (based on viscosity)，Physical form 75-85% 脱乙酰化）的糖胺的摩尔数。
   非常感谢。

参考的文献为 Xin Wei, Juan Cruz, and Waldemar Gorski*， Integration of Enzymes and Electrodes: Spectroscopic and Electrochemical Studies of Chitosan-Enzyme Films， Anal. Chem. 2002, 74, 5039-5046。

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