| 查看: 4426 | 回复: 7 | |||
2429650199新虫 (正式写手)
|
[交流]
关于用EDC/NHS活化抗体上羧基的最佳条件的问题 已有4人参与
|
|
各位虫友早上好，实验一直遇到瓶颈，感觉好烦躁，做电化学免疫传感器时为了将抗体结合在氨基化的无机材料上，用EDC/NHS活化的方法活化羧基，但是测DPV总感觉结合效果不好，不知道是不是活化条件没控制好，还是孵化过程有问题，希望做过相关工作并成效卓著的虫友能给个建议 发自小木虫IOS客户端 |
回复此楼» 猜你喜欢
请问谁有2025最新影响因子Excel表？金币求助！
已经有53人回复
-
氯化钠与有机磺酸分离
已经有1人回复
-
分析化学论文润色/翻译怎么收费?
已经有140人回复
-
瑞典-博后-固态电池-粘结剂-锂离子电池方向
已经有53人回复
-
广东省面上有什么动态么，啥时候公布呀？
已经有1人回复
-
高盐废水电催化氧化，氯离子存在下活性氯副产物、阳极选型的工程困惑
已经有0人回复
-
固相反应中颗粒形貌变化计算模拟学习求助
已经有0人回复
-
电催化氧化技术处理高盐难降解废水具备独特优势
已经有0人回复
» 本主题相关商家推荐: (我也要在这里推广)
2429650199
新虫 (正式写手)
- 应助: 0 (幼儿园)
- 金币: 63.3
- 散金: 1466
- 红花: 1
- 帖子: 406
- 在线: 41.4小时
- 虫号: 3722130
- 注册: 2015-03-09
- 专业: 生化分析及生物传感
2楼2018-05-15 11:55:32
2429650199
新虫 (正式写手)
- 应助: 0 (幼儿园)
- 金币: 63.3
- 散金: 1466
- 红花: 1
- 帖子: 406
- 在线: 41.4小时
- 虫号: 3722130
- 注册: 2015-03-09
- 专业: 生化分析及生物传感
3楼2018-05-15 11:55:41
★
小木虫: 金币+0.5, 给个红包，谢谢回帖
小木虫: 金币+0.5, 给个红包，谢谢回帖
|
本帖内容被屏蔽 |
4楼2018-05-18 19:40:21
★
小木虫: 金币+0.5, 给个红包，谢谢回帖
小木虫: 金币+0.5, 给个红包，谢谢回帖
|
Follow this paper to try periodate method to make orientated conjugation. Good luck. Sara Puertas, Pilar Batalla, María Moros, Ester Polo, Pablo del Pino, José M. Guisán, Valeria Grazú, Jesús M. de la Fuente. Taking Advantage of Unspecific Interactions to Produce Highly Active Magnetic Nanoparticle−Antibody Conjugates. ACS Nano. 2011, 5(6): 4521-4528. Just try the old classcial style (change its magnetic beads to your electrode surface) Immobilization via the Carbohydrates Moieties of the Antibody. One milliliter of an antibody solution 1 mg/mL in 10 mM sodium phosphate pH 7.0 was incubated with 100 μL of 0.1 M NaIO4 (prepared in water) during 2 h at 4C and preserved from light. Then, the oxidized antibody was purified by Sephadex G-25 gel filtration column with 10 mM sodium phosphate pH 8.0. Aliquots of 10 mg of aminated nanoparticles were incubated during 2 h at 37 C with 1 mL of a 100 μg/mL solution of oxidized antibody in 10 mM sodium phosphate pH 8.0. Then, NaCNBH3 was added in order to have a final concentration of 0.25 M. After 30 min at 37 C, the functionalized nanoparticles were washedthree times with10 mM sodium phosphate pH 8.0. Finally, the remaining amine groups of the nanoparticles were blocked by incubation with 1 mL BSA 1% in 10 mM MES pH 6.1 for 16 h at 25 C and were stored at 4 C until used. |
5楼2018-05-20 05:05:28
★
小木虫: 金币+0.5, 给个红包，谢谢回帖
小木虫: 金币+0.5, 给个红包，谢谢回帖
|
" Then, the oxidized antibody was purified by Sephadex G-25 gel filtration column with 10 mM sodium phosphate pH 8.0. " This step can be replaced using Zeba™ Spin Desalting Columns "https://www.thermofisher.com/ord ... ct/89882". Much simple and fast without diluting your antibody. |
6楼2018-05-20 05:16:30
7楼2018-05-22 10:23:46
8楼2018-06-29 16:05:39
20