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【答案】应助回帖

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感谢参与，应助指数 +1
xiandongzeng: 金币+5, ★★★★★最佳答案, 谢谢 2018-01-09 12:35:43
心静_依然: LS-EPI+1, 感谢应助 2018-01-11 10:30:10
Layer-by-Layer Assembly of Hemoglobin and DNA Functionalized Carbon Nanotubes on Glassy Carbon Electrode: Direct Electrochemistry and Electrocatalysis
作者:Zeng, XD (Zeng, Xiandong)[ 1 ] ; Liu, J (Liu, Jie)[ 1 ] ; Kong, S (Kong, Shu)[ 1 ] ; Zhang, ZP (Zhang, Ziping)[ 2 ]
ELECTROCHEMISTRY
卷: 83  期: 11  页: 979-983
DOI: 10.5796/electrochemistry.83.979
出版年: NOV 2015
查看期刊影响力
摘要
Layer-by-layer assembly of hemoglobin (Hb) with DNA functionalized singlewall carbon nanotubes (DNA-SWCNTs) was achieved on glassy carbon electrode surface based on the electrostatic attraction between positively charged Hb and negatively charged DNA-SWCNTs hybrids. Cyclic voltammogram of (Hb/DNA-SWCNTs)(n) films modified electrodes showed a pair of well-defined and quasireversible redox peaks for Hb Fe(III)/Fe(II), indicating that direct electrochemistry of Hb was achieved. The dependence of the formal potential on solution pH indicated that one-proton transfer was coupled to each electron transfer in the direct electron transfer reaction. Additionally, Hb in the multilayer films retained its bioactivity and showed excellent electrocatalytic activity toward H2O2, suggesting that such multilayer films could be used as reagentless biosensors. The electrocatalytic current values were linear with increasing concentration of H2O2 in a wide range of 1-80 mu M, with a low detection limit of 0.2 mu M. The layer-by-layer assembly of enzymes with DNA-SWCNTs hybrids provided a general and useful way to construct sensitive biosensors without using mediators. On the other hand, this would be used as an easily prepared experimental model to fabricate functional nanostructured biointerfaces. (C) The Electrochemical Society of Japan, All rights reserved.
关键词
作者关键词:Layer-by-Layer Assembly; Carbon Nanotubes; Nanostructured Biointerfaces; Bioelectrochemistry
KeyWords PlusYROLYTIC-GRAPHITE ELECTRODES; HEME-PROTEINS; CONTAINING ENZYMES; FILMS; BIOELECTROCHEMISTRY; MYOGLOBIN; NANOPARTICLES; BIOSENSOR; FABRICATION; DENDRIMERS
作者信息
通讯作者地址: Zeng, XD (通讯作者)
              Shenzhen Nanshan Ctr Dis Control & Prevent, Shenzhen 518054, Peoples R China.
地址:
              [ 1 ] Shenzhen Nanshan Ctr Dis Control & Prevent, Shenzhen 518054, Peoples R China
显示更多        [ 2 ] Yantai Univ, Coll Life Sci, Yantai 264005, Peoples R China
电子邮件地址:zengxiandong2006@126.com; szjieliu@gmail.com
基金资助致谢
基金资助机构        授权号
National Natural Science Foundation of China        
21305121
查看基金资助信息   
出版商
ELECTROCHEMICAL SOC JAPAN, ARUSUICHIGAYA202, 4-8-30, KUDANMINAMI, CHIYODA-KU, TOKYO, 102-0074, JAPAN
类别 / 分类
研究方向:Electrochemistry
Web of Science 类别:Electrochemistry
文献信息
文献类型:Article
语种:English
入藏号: WOS:000364614800006
ISSN: 1344-3542
期刊信息
Impact Factor (影响因子): Journal Citation Reports
其他信息
IDS 号: CV9NV
Web of Science 核心合集中的 "引用的参考文献": 45
Web of Science 核心合集中的 "被引频次": 0
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