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ÇóÖúWOSÈë²ØºÅ, Layer-by-Layer Assembly of Hemoglobin and DNA Functionalized Carbon Nanotubes on Glassy Carbon Electrode: Direct Electrochemistry and Electrocatalysis, Electrochemistry, 2015, 83(11): 979¨C983

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xiandongzeng: ½ð±Ò+5, ¡ï¡ï¡ï¡ï¡ï×î¼Ñ´ð°¸, лл 2018-01-09 12:35:43
Ðľ²_ÒÀÈ»: LS-EPI+1, ¸ÐлӦÖú 2018-01-11 10:30:10
Layer-by-Layer Assembly of Hemoglobin and DNA Functionalized Carbon Nanotubes on Glassy Carbon Electrode: Direct Electrochemistry and Electrocatalysis
×÷Õß:Zeng, XD (Zeng, Xiandong)[ 1 ] ; Liu, J (Liu, Jie)[ 1 ] ; Kong, S (Kong, Shu)[ 1 ] ; Zhang, ZP (Zhang, Ziping)[ 2 ]
ELECTROCHEMISTRY
¾í: 83  ÆÚ: 11  Ò³: 979-983
DOI: 10.5796/electrochemistry.83.979
³ö°æÄê: NOV 2015
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Layer-by-layer assembly of hemoglobin (Hb) with DNA functionalized singlewall carbon nanotubes (DNA-SWCNTs) was achieved on glassy carbon electrode surface based on the electrostatic attraction between positively charged Hb and negatively charged DNA-SWCNTs hybrids. Cyclic voltammogram of (Hb/DNA-SWCNTs)(n) films modified electrodes showed a pair of well-defined and quasireversible redox peaks for Hb Fe(III)/Fe(II), indicating that direct electrochemistry of Hb was achieved. The dependence of the formal potential on solution pH indicated that one-proton transfer was coupled to each electron transfer in the direct electron transfer reaction. Additionally, Hb in the multilayer films retained its bioactivity and showed excellent electrocatalytic activity toward H2O2, suggesting that such multilayer films could be used as reagentless biosensors. The electrocatalytic current values were linear with increasing concentration of H2O2 in a wide range of 1-80 mu M, with a low detection limit of 0.2 mu M. The layer-by-layer assembly of enzymes with DNA-SWCNTs hybrids provided a general and useful way to construct sensitive biosensors without using mediators. On the other hand, this would be used as an easily prepared experimental model to fabricate functional nanostructured biointerfaces. (C) The Electrochemical Society of Japan, All rights reserved.
¹Ø¼ü´Ê
×÷Õ߹ؼü´Ê:Layer-by-Layer Assembly; Carbon Nanotubes; Nanostructured Biointerfaces; Bioelectrochemistry
KeyWords PlusYROLYTIC-GRAPHITE ELECTRODES; HEME-PROTEINS; CONTAINING ENZYMES; FILMS; BIOELECTROCHEMISTRY; MYOGLOBIN; NANOPARTICLES; BIOSENSOR; FABRICATION; DENDRIMERS
×÷ÕßÐÅÏ¢
ͨѶ×÷ÕßµØÖ·: Zeng, XD (ͨѶ×÷Õß)
              Shenzhen Nanshan Ctr Dis Control & Prevent, Shenzhen 518054, Peoples R China.
µØÖ·:
              [ 1 ] Shenzhen Nanshan Ctr Dis Control & Prevent, Shenzhen 518054, Peoples R China
ÏÔʾ¸ü¶à        [ 2 ] Yantai Univ, Coll Life Sci, Yantai 264005, Peoples R China
µç×ÓÓʼþµØÖ·:zengxiandong2006@126.com; szjieliu@gmail.com
»ù½ð×ÊÖúÖÂл
»ù½ð×ÊÖú»ú¹¹        ÊÚȨºÅ
National Natural Science Foundation of China        
21305121
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ELECTROCHEMICAL SOC JAPAN, ARUSUICHIGAYA202, 4-8-30, KUDANMINAMI, CHIYODA-KU, TOKYO, 102-0074, JAPAN
Àà±ð / ·ÖÀà
Ñо¿·½Ïò:Electrochemistry
Web of Science Àà±ð:Electrochemistry
ÎÄÏ×ÐÅÏ¢
ÎÄÏ×ÀàÐÍ:Article
ÓïÖÖ:English
Èë²ØºÅ: WOS:000364614800006
ISSN: 1344-3542
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Impact Factor (Ó°ÏìÒò×Ó): Journal Citation Reports
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IDS ºÅ: CV9NV
Web of Science ºËÐĺϼ¯ÖÐµÄ "ÒýÓõIJο¼ÎÄÏ×": 45
Web of Science ºËÐĺϼ¯ÖÐµÄ "±»ÒýƵ´Î": 0
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