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黑曲霉-鼠李糖苷酶高产菌株的选育 摘 要:本文首次利用Davis方法制备的透明圈筛选α-鼠李糖苷酶高产菌株。用甲基磺酸乙酯对出芽8 h的黑曲霉孢子进行诱变处理，用透明圈法初筛出的菌株中，产量提高40%以上的突变菌株占11％；用摇瓶培养对初筛出的菌株进行两轮复筛α-鼠李糖苷酶高产突变株T-226，摇瓶培养α-鼠李糖苷酶活达373.4 U/mL，比出发菌株提高了22.7%。对该高产突变株进行5 L罐发酵实验，在发酵第84 h测得α-鼠李糖苷酶活为631.9 U/mL。用新建立的方法选育高产α-鼠李糖苷酶的高产菌株，不仅具有较高的筛选效率，还具有良好的准确性。 Screening of α-Rhamnosidase high-yield strain from Aspergillus niger Abstract: In this study, Davis method of plate transparent circle were introduce firstly to screen α-Rhamnosidase high-yield strain. Ethyl methane sulphonate was used to treat the Aspergillus niger sprouted spores of 8 h . Then mutants were pre-screened by plate transparent circle methods. The results showed that about 11％mutants had more than 40％ higher yield of α-rhamnosidase than the original strain. A high-yeild strain, T-226 with the highest α-rhamnosidase activity of 373.4 U/mL was finally selected from these potential high-yeild mutants after rescreened by shake flask fermentation twice. When the T-226 strain was fermented in 5 L bioreactor, the enzyme activity could reach to 631.9 U/mL after 84 h. Thus, the established screening method is highly efficient to isolate α-rhamnosidase high-yeild mutant of A. niger. |
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rachel-oy
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telomerase(金币+5,VIP+0):thanks 4-23 17:45
telomerase(金币+5,VIP+0):thanks 4-23 17:45
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Screening of α-Rhamnosidase high-yield strain from Aspergillus niger Abstract: Davis method of plate transparent circle were firstly introduced to screen α-Rhamnosidase high-yield strain in this paper. After being mutagenized to Aspergillus niger sprouted spores for 8 h with Ethyl methane sulphonate , and pre-screened by plate transparent circle methods , the mutants which output increased by 40% account for 11%. A high-yeild strain, T-226 with the highest α-rhamnosidase activity of 373.4 U/mL was finally selected from these potential high-yeild mutants after rescreened by shaking flask fermentation twice. When the T-226 strain was fermented in 5 L bioreactor, the enzyme activity could reach to 631.9 U/mL after 84 h. Thus, the established screening method is highly efficient to isolate α-rhamnosidase high-yeild mutant of A. niger. 主要改了下前两句，不知道您感觉怎样。 |
4楼2009-03-09 17:19:37
caitikuan
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chenyyy(金币+15,VIP+0):谢谢... 3-1 09:22
chenyyy(金币+15,VIP+0):谢谢... 3-1 09:22
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the selection of a high-yield α-Rhamnosidase strain from Aspergillus niger Abstract: In this study, Davis method of plate transparent circle were introduced firstly screening α-Rhamnosidase high-yield strain. 8-hour sporo-sprouted Aspergillus niger were mutagenized by ethyl methane sulphonate and selected via transparent circle. From this 11% mutans had more than 40％ higher yield of α-rhamnosidase than the original strain. A high-yeild strainT-226 with the highest α-rhamnosidase activity of 373.4 U/mL was finally selected from these potential high-yeild mutants after rescreened by shake-flask culture twice. When the T-226 strain was fermented in 5 L bioreactor, the enzyme activity could reach to 631.9 U/mL after 84 h. Thus, the established screening method is highly efficient to isolate α-rhamnosidase high-yeild mutant of A. niger. |
2楼2009-03-01 02:15:41
hust_gaoyi
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chenyyy(金币+10,VIP+0):谢谢...你的建议. 3-1 13:37
chenyyy(金币+10,VIP+0):谢谢...你的建议. 3-1 13:37
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The breeding of a high-yield α-Rhamnosidase strain from Aspergillus niger Abstract: In this study, the transparent circle by Davis method was firstly introduced screening α-Rhamnosidase high-yield strain. The sporo-sprouted Aspergillus niger 8-hour were mutagenized by ethyl methane sulphonate and selected via transparent circle. 11% of the mutans yield 40% higher of α-rhamnosidase than the original strain.... BTW:我不是这个专业的，专有名词是参照楼上的。就是对楼上的一些表达上，以及语法上做了一点修改！ |
3楼2009-03-01 12:29:49