| 查看: 461 | 回复: 3 | |||
| 当前主题已经存档。 | |||
[交流]
翻译求助:摘要修改
|
|||
|
黑曲霉-鼠李糖苷酶高产菌株的选育 摘 要:本文首次利用Davis方法制备的透明圈筛选α-鼠李糖苷酶高产菌株。用甲基磺酸乙酯对出芽8 h的黑曲霉孢子进行诱变处理，用透明圈法初筛出的菌株中，产量提高40%以上的突变菌株占11％；用摇瓶培养对初筛出的菌株进行两轮复筛α-鼠李糖苷酶高产突变株T-226，摇瓶培养α-鼠李糖苷酶活达373.4 U/mL，比出发菌株提高了22.7%。对该高产突变株进行5 L罐发酵实验，在发酵第84 h测得α-鼠李糖苷酶活为631.9 U/mL。用新建立的方法选育高产α-鼠李糖苷酶的高产菌株，不仅具有较高的筛选效率，还具有良好的准确性。 Screening of α-Rhamnosidase high-yield strain from Aspergillus niger Abstract: In this study, Davis method of plate transparent circle were introduce firstly to screen α-Rhamnosidase high-yield strain. Ethyl methane sulphonate was used to treat the Aspergillus niger sprouted spores of 8 h . Then mutants were pre-screened by plate transparent circle methods. The results showed that about 11％mutants had more than 40％ higher yield of α-rhamnosidase than the original strain. A high-yeild strain, T-226 with the highest α-rhamnosidase activity of 373.4 U/mL was finally selected from these potential high-yeild mutants after rescreened by shake flask fermentation twice. When the T-226 strain was fermented in 5 L bioreactor, the enzyme activity could reach to 631.9 U/mL after 84 h. Thus, the established screening method is highly efficient to isolate α-rhamnosidase high-yeild mutant of A. niger. |
回复此楼» 猜你喜欢
312求调剂
已经有5人回复
-
0703调剂，一志愿天津大学319分
已经有9人回复
-
085600材料与化工301分求调剂院校
已经有10人回复
-
专硕0854初试考材科基，求调剂
已经有3人回复
-
求调剂
已经有8人回复
-
085600，320分求调剂
已经有17人回复
-
297分083200求助
已经有3人回复
-
求调剂
已经有7人回复
-
一志愿北交大材料工程总分358求调剂
已经有5人回复
-
一志愿南京航空航天大学 材料与化工329分求调剂
已经有7人回复
caitikuan
木虫 (小有名气)
- 应助: 0 (幼儿园)
- 金币: 2243.1
- 帖子: 229
- 在线: 102小时
- 虫号: 518882
- 注册: 2008-03-05
- 性别: GG
- 专业: 器官移植与移植免疫
★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★
chenyyy(金币+15,VIP+0):谢谢... 3-1 09:22
chenyyy(金币+15,VIP+0):谢谢... 3-1 09:22
|
the selection of a high-yield α-Rhamnosidase strain from Aspergillus niger Abstract: In this study, Davis method of plate transparent circle were introduced firstly screening α-Rhamnosidase high-yield strain. 8-hour sporo-sprouted Aspergillus niger were mutagenized by ethyl methane sulphonate and selected via transparent circle. From this 11% mutans had more than 40％ higher yield of α-rhamnosidase than the original strain. A high-yeild strainT-226 with the highest α-rhamnosidase activity of 373.4 U/mL was finally selected from these potential high-yeild mutants after rescreened by shake-flask culture twice. When the T-226 strain was fermented in 5 L bioreactor, the enzyme activity could reach to 631.9 U/mL after 84 h. Thus, the established screening method is highly efficient to isolate α-rhamnosidase high-yeild mutant of A. niger. |
2楼2009-03-01 02:15:41
hust_gaoyi
金虫 (正式写手)
- 翻译EPI: 18
- 应助: 0 (幼儿园)
- 金币: 1266.1
- 沙发: 1
- 帖子: 575
- 在线: 87.7小时
- 虫号: 488206
- 注册: 2007-12-29
- 专业: 计算机软件
★ ★ ★ ★ ★ ★ ★ ★ ★ ★
chenyyy(金币+10,VIP+0):谢谢...你的建议. 3-1 13:37
chenyyy(金币+10,VIP+0):谢谢...你的建议. 3-1 13:37
|
The breeding of a high-yield α-Rhamnosidase strain from Aspergillus niger Abstract: In this study, the transparent circle by Davis method was firstly introduced screening α-Rhamnosidase high-yield strain. The sporo-sprouted Aspergillus niger 8-hour were mutagenized by ethyl methane sulphonate and selected via transparent circle. 11% of the mutans yield 40% higher of α-rhamnosidase than the original strain.... BTW:我不是这个专业的，专有名词是参照楼上的。就是对楼上的一些表达上，以及语法上做了一点修改！ |
3楼2009-03-01 12:29:49
rachel-oy
金虫 (正式写手)
- 应助: 5 (幼儿园)
- 金币: 1832.1
- 散金: 41
- 红花: 1
- 沙发: 1
- 帖子: 515
- 在线: 102.4小时
- 虫号: 551284
- 注册: 2008-04-26
- 性别: MM
- 专业: 金属结构材料
★ ★ ★ ★ ★
telomerase(金币+5,VIP+0):thanks 4-23 17:45
telomerase(金币+5,VIP+0):thanks 4-23 17:45
|
Screening of α-Rhamnosidase high-yield strain from Aspergillus niger Abstract: Davis method of plate transparent circle were firstly introduced to screen α-Rhamnosidase high-yield strain in this paper. After being mutagenized to Aspergillus niger sprouted spores for 8 h with Ethyl methane sulphonate , and pre-screened by plate transparent circle methods , the mutants which output increased by 40% account for 11%. A high-yeild strain, T-226 with the highest α-rhamnosidase activity of 373.4 U/mL was finally selected from these potential high-yeild mutants after rescreened by shaking flask fermentation twice. When the T-226 strain was fermented in 5 L bioreactor, the enzyme activity could reach to 631.9 U/mL after 84 h. Thus, the established screening method is highly efficient to isolate α-rhamnosidase high-yeild mutant of A. niger. 主要改了下前两句，不知道您感觉怎样。 |
4楼2009-03-09 17:19:37
