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线粒体DNA 已有1人参与
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长链pcr的原理是什么？ 发自小木虫IOS客户端 |
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Rose56882
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2楼2017-11-28 09:36:06
Rose56882
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Efficient Long PCR results from the use of two polymerases: a non-proofreading polymerase is the main polymerase in the reaction, and a proofreading polymerase (3' to 5' exo) is present at a lower concentration. Following the results of Cheng et al. (1) we have had success using Tth (ABI/Perkin-Elmer) as the main-component polymerase and Vent (New England Biolabs) as the fractional-component polymerase. Other combinations of proofreading and non-proofreading polymerases have been used successfully for many applications. The buffer listed below works well with Tth and Vent, but not with others. If you are interested in using other polymerases make sure that you use compatible buffers. 发自小木虫IOS客户端 |
3楼2017-11-28 09:43:32
Rose56882
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感谢参与，应助指数 +1
1125科研王子: 金币+500 2017-11-28 09:45:34
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感谢参与，应助指数 +1
1125科研王子: 金币+500 2017-11-28 09:45:34
silicare: 金币-1, 违规存档, 不要转移金币，下不为例 2017-11-28 12:05:29
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Efficient Long PCR results from the use of two polymerases: a non-proofreading polymerase is the main polymerase in the reaction, and a proofreading polymerase (3' to 5' exo) is present at a lower concentration. Following the results of Cheng et al. (1) we have had success using Tth (ABI/Perkin-Elmer) as the main-component polymerase and Vent (New England Biolabs) as the fractional-component polymerase. Other combinations of proofreading and non-proofreading polymerases have been used successfully for many applications. The buffer listed below works well with Tth and Vent, but not with others. If you are interested in using other polymerases make sure that you use compatible buffers. 发自小木虫IOS客户端 |
4楼2017-11-28 09:44:54