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艾德科技（北京）有限公司

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表观遗传学（m6A RNA甲基化、RNA甲基化、DNA甲基化、DNA羟甲基、DNA甲酰基、DNA羧基），分子生物学，免疫生物学（一抗、二抗），细胞生物学，实验仪器学（核酸提起仪、超微量分光光度计）等等产品与技术服务

北京市昌平区中关村生命科学园东60米艾德园

联系人: Peter Chen / 邮箱: 1951545998@qq.com / QQ: 1513545070 / 电话: 0313-5935521 / 手机: 18911529660

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[商家供应] 为测序而设计---DNA甲基化极易修饰试剂盒（测序版）-A-P-1054

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NA甲基化极易修饰试剂盒货号：A-P-1054

BisulFlash™ DNA Bisulfite Conversion Easy Kit

背景资料：DNA methylation is essential in regulating gene expression in nearly all biological processes including development, growth, and differentiation. Gene/region-specific or genome-wide analysis of DNA methylation or 5-methylcytosine (5-mC) could provide valuable information for discovering epigenetic markers used for disease diagnosis, and potential targets used for therapeutics. By treating DNA with bisulfite, cytosine residues are deaminated to uracil while leaving 5-methylcytosine intact, so that methylated cytosine can be properly identified via PCR, array, bisulfite sequencing, or next generation sequencing.

产品描述：This kit contains all reagents necessary for a fast bisulfite conversion in a high throughput format. With the unique conversion mix solution, DNA denaturation status is sustained throughout the entire bisulfite conversion process, thereby enabling all unmethylated cytosines to be successfully converted to uracil. Desulfonation and clean-up of the converted DNA is performed using DNA purification columns. High yield, converted DNA can be obtained and used for various downstream applications including PCR, array, and next generation sequencing.

产品特点：

1· Fast Procedure: The entire process can be completed within 1 hour；

2·Streamlined: Concurrently processes the DNA denaturation and C to T conversion steps without the need for a separate DNA denaturation step；

3.Convenient : The bisulfite conversion reagent is in a stable, pre-mixed liquid solution format, eliminating the need for powder-based preparation；

4.Complete Conversion : Completely converts unmethylated cytosine into uracil (>99.9%) with negligible inappropriate or erroneous conversion of methylcytosine to thymine (<0.1%)；

5.Robust: Simple, reliable, and consistent reaction conditions with an easy-to-follow protocol and high yield。
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