24小时热门版块排行榜    

查看: 941  |  回复: 4
当前主题已经存档。
当前只显示满足指定条件的回帖,点击这里查看本话题的所有回帖

恒星年

银虫 (正式写手)

[交流] 【求助】请教玻璃材料的修饰的问题(用于固定蛋白)

AMPCPG(Aminopropyl-tethered controlled pore glass beads) 是指经过如何修饰的玻璃材料啊?是用来固定蛋白质的,有没有人能介绍下,不胜感激!能介绍下,不胜感激!

[ Last edited by wgcui on 2009-2-8 at 19:55 ]
回复此楼
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖

whn732

禁虫 (正式写手)

★
wgcui(金币+1,VIP+0):请在具体些。希望多多讨论! 2-11 22:44
是否可以使用强碱进行表面处理,使表面增加羟基,然后进行表面接枝,具体怎么接枝,查一下文献,相关接枝方法的报道还是很多的吧。
4楼2009-02-11 20:41:38
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖
查看全部 5 个回答

wgcui

铁杆木虫 (著名写手)

★ ★
yusen_1982(金币+1,VIP+0):谢谢应助,好帖子 2-8 20:05
恒星年(金币+1,VIP+0):我就是要参考这篇文献的,但是不知道他用的APMCPG材料是什么样的,在国内叫什么?在哪买,我想买这种玻璃片。谢谢 2-12 11:07
给你发一篇文献:

Effect of Linker for Immobilization of Glutathione on BSA-Assembled Controlled Pore Glass Beads



Controlled pore glass bead was modified with bovine serum albumin (BSA), and glutathione (GSH) was immobilized through three kinds of linkers on top of BSA. Bis(3-sulfo-N-hydroxysuccinimide suberate) sodium salt (BS3), N-hydroxysuccinimide 3-(2-pyridyldithio)propionate (SPDP), or N-hydroxysuccinimide 4-maleimidobutyrate (GMBS) was introduced into the BSA-bound matrix. Subsequently, GSH was immobilized by addition of thiol side chain into the maleimido moiety, replacing a disulfide group, or formation of an amide group upon releasing 3-sulfo-N-hydroxysuccimide group. It was observed that conjugation methodology
played a critical role for activity of the immobilized GSH. SDS-PAGE chromatogram showed that the matrix of glutathione immobilized on BSA through GMBS manifested high selectivity towards glutathione-Stransferase (GST) in cell lysate.

链接:
http://www.namipan.com/d/f6cb83d ... 551e06d004cdadf1200


Interaction of Sucrose Esters with Skim Milk Proteins as Characterized by Affinity Chromatography

链接:
http://www.namipan.com/d/a093535 ... ed0ba3658322a2e0900

我想你问题的答案可以解决吧。
2楼2009-02-08 19:53:41
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖

yew1100

木虫 (小有名气)


深奥,不过加一下人气
3楼2009-02-11 11:56:58
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖

恒星年

银虫 (正式写手)

我就是要做1楼文献上说的那种,但是那里面的材料就是AMPCPG,他用的是玻璃珠,但是我想用玻璃片,所以我要买类似修饰的玻片。但是我不明白AMP是什么修饰,我感觉像是氨基烷化,不敢不确定。不知道有没有人用过这个材料。
5楼2009-02-12 11:04:48
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖
普通表情 龙 兔 虎 猫 高级回复 (可上传附件)
最具人气热帖推荐 [查看全部] 作者 回/看 最后发表
[教师之家] 售SCI一区T0P文章,我:8.O.55.1.O.5.4,科目全,可+急 +4 1Nb0IVlmV6v0 2026-11-11 4/200 2026-11-13 11:18 by Equinoxhua
[硕博家园] 售SCI-T0P文章,我:8O.5.5.1.O.54,科目齐全,可+急 +6 zSbvVX9JFl9J 2026-11-09 9/450 2026-11-13 11:13 by Equinoxhua
[考研] 售一区SCI文章T0P,我:8O.551.O54,科目全,可十急 +8 zSbvVX9JFl9J 2026-11-09 9/450 2026-11-13 11:11 by Equinoxhua
[考博] 27博士申请 +5 白白白ly 2026-11-09 5/250 2026-11-13 05:01 by wlf1982
[基金申请] 售SCI一区T0P文章,我:8.O55.1.O.54,科目全,可十急 +4 Z7qfCsdIiD1T 2026-11-11 5/250 2026-11-12 11:44 by HajghKOMynmz
[考研] 售SCI一区文章,我:8.O.55.1.O.54,科目齐全,可伽急 +5 Z7qfCsdIiD1T 2026-11-10 8/400 2026-11-12 11:22 by HajghKOMynmz
[教师之家] 售SCI一区文章,我:8.O.551.O.5.4,科目全,可伽急 +4 Z7qfCsdIiD1T 2026-11-10 9/450 2026-11-12 11:22 by HajghKOMynmz
[考研] 售SCI一区T0P文章,我:8O.55.1.O.54,科目全,可伽急 +4 Z7qfCsdIiD1T 2026-11-10 4/200 2026-11-12 11:22 by HajghKOMynmz
[硕博家园] 售SCI-T0P文章,我:8O.5.5.1.O.54,科目齐全,可+急 +5 Z7qfCsdIiD1T 2026-11-10 8/400 2026-11-12 11:20 by HajghKOMynmz
[考研] 售SCI一区T0P文章,我:8O.55.1.O.54,科目全,可伽急 +4 G2rhSvqvhNzl 2026-11-10 7/350 2026-11-12 10:49 by HajghKOMynmz
[硕博家园] 售SCI文章,我:8O.5.5.1O.54,科目全,可十急 +4 czAiolzR0IJC 2026-11-10 8/400 2026-11-12 10:49 by HajghKOMynmz
[公派出国] 售SCI-T0P文章,我:8O.5.5.1.O.54,科目齐全,可+急 +4 C6CRn66RYYXS 2026-11-10 8/400 2026-11-12 10:27 by HajghKOMynmz
[教师之家] 售SCI一区T0P文章,我:8.O.55.1.O54,科目全,可伽急 +7 FChKX8meu1ds 2026-11-09 12/600 2026-11-12 09:43 by HajghKOMynmz
[教师之家] 售SCI一区T0P文章,我:8.O55.1.O.54,科目全,可十急 +8 zSbvVX9JFl9J 2026-11-09 12/600 2026-11-12 09:21 by HajghKOMynmz
[考研] 售SCI一区T0P文章,我:8.O.55.1.O.54,科目齐全,可+急 +4 C6CRn66RYYXS 2026-11-10 6/300 2026-11-12 06:03 by 9fOTqj074tIg
[教师之家] 售SCI文章,我:8O5.5.1.O.54,科目齐全,可+急 +4 C6CRn66RYYXS 2026-11-10 4/200 2026-11-12 06:03 by 9fOTqj074tIg
[公派出国] 售SCI-T0P文章,我:8O.5.5.1.O.54,科目齐全,可+急 +5 zSbvVX9JFl9J 2026-11-09 8/400 2026-11-12 04:57 by 9fOTqj074tIg
[硕博家园] 售SCI文章,我:8O.5.5.1O.54,科目全,可十急 +6 0TEMgxdT1eIw 2026-11-09 9/450 2026-11-12 04:57 by 9fOTqj074tIg
[考研] 售一区SCI文章T0P,我:8O.551.O54,科目全,可十急 +3 Z7qfCsdIiD1T 2026-11-10 4/200 2026-11-12 02:23 by 9fOTqj074tIg
[考研] 售SCI一区T0P文章,我:8O.55.1.O.54,科目全,可伽急 +3 5sJFDKp2kwuI 2026-11-06 12/600 2026-11-09 06:32 by l48hFd8Nwae2
信息提示
请填处理意见