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【求助】请教玻璃材料的修饰的问题（用于固定蛋白）
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AMPCPG（Aminopropyl-tethered controlled pore glass beads) 是指经过如何修饰的玻璃材料啊？是用来固定蛋白质的，有没有人能介绍下，不胜感激！能介绍下，不胜感激！ [ Last edited by wgcui on 2009-2-8 at 19:55 ] |
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wgcui
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yusen_1982(金币+1,VIP+0):谢谢应助,好帖子 2-8 20:05
恒星年(金币+1,VIP+0):我就是要参考这篇文献的，但是不知道他用的APMCPG材料是什么样的，在国内叫什么？在哪买，我想买这种玻璃片。谢谢 2-12 11:07
yusen_1982(金币+1,VIP+0):谢谢应助,好帖子 2-8 20:05
恒星年(金币+1,VIP+0):我就是要参考这篇文献的，但是不知道他用的APMCPG材料是什么样的，在国内叫什么？在哪买，我想买这种玻璃片。谢谢 2-12 11:07
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给你发一篇文献： Effect of Linker for Immobilization of Glutathione on BSA-Assembled Controlled Pore Glass Beads Controlled pore glass bead was modified with bovine serum albumin (BSA), and glutathione (GSH) was immobilized through three kinds of linkers on top of BSA. Bis(3-sulfo-N-hydroxysuccinimide suberate) sodium salt (BS3), N-hydroxysuccinimide 3-(2-pyridyldithio)propionate (SPDP), or N-hydroxysuccinimide 4-maleimidobutyrate (GMBS) was introduced into the BSA-bound matrix. Subsequently, GSH was immobilized by addition of thiol side chain into the maleimido moiety, replacing a disulfide group, or formation of an amide group upon releasing 3-sulfo-N-hydroxysuccimide group. It was observed that conjugation methodology played a critical role for activity of the immobilized GSH. SDS-PAGE chromatogram showed that the matrix of glutathione immobilized on BSA through GMBS manifested high selectivity towards glutathione-Stransferase (GST) in cell lysate. 链接： http://www.namipan.com/d/f6cb83d ... 551e06d004cdadf1200 Interaction of Sucrose Esters with Skim Milk Proteins as Characterized by Affinity Chromatography 链接： http://www.namipan.com/d/a093535 ... ed0ba3658322a2e0900 我想你问题的答案可以解决吧。 |
2楼2009-02-08 19:53:41
yew1100
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3楼2009-02-11 11:56:58
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