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雨中背水

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[求助] 如何快速检测敲除的植物是否发生突变？已有1人参与

听闻High-Efficiency gRNA-Cas9-Puro Plasmid (linear) Assembly Kit可以快速检测敲除样品，就想问问有没有用过？效果如何

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晋鹏

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【答案】应助回帖

感谢参与，应助指数 +1

这种技术相当热门，最近几年已经有很多这样的文章报道，你可以下载看看。

CRISPR/Cas9 mediated genome editing is a powerful technique that allows you to create knock-in, knock-out, gene up-regulation/repression, and mutations of any gene, in any cell, in a highly targeted manner.

The CRISPR/Cas system mainly consists of two major components: the Cas nuclease Cas9 and the specific guide RNA (gRNA). They can form a very stable ribonucleoprotein (RNP) complex. Once a specific DNA region is matched by the gRNA carrying Cas9, the target DNA can be cleaved into double strand break. Cas9 RNP complex can be introduced into cells directly and act immediately after it enters the cell without transcription and translation. By fusion with different functional domain, CRISPR/Cas9 and its mutants have been used in a broad gene editing fields (knock-out, knock-in, gene up/down, genome purification and visualization et. al.).