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Preliminary Proteomic Analysis of A549 Cells Infected with Avian Influenza Virus H7N9 and Influenza A Virus H1N1, Plos One , 2016 , 11 (5) : e0156017 лл |
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baiyuefei
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Preliminary Proteomic Analysis of A549 Cells Infected with Avian Influenza Virus H7N9 and Influenza A Virus H1N1 作者  ing, XM (Ding, Xiaoman)[ 1 ] ; Lu, JH (Lu, Jiahai)[ 1 ] ; Yu, RX (Yu, Ruoxi)[ 2 ] ; Wang, X (Wang, Xin)[ 3 ] ; Wang, T (Wang, Ting)[ 4 ] ; Dong, FY (Dong, Fangyuan)[ 3 ] ; Peng, B (Peng, Bo)[ 3 ] ; Wu, WH (Wu, Weihua)[ 3 ] ; Liu, H (Liu, Hui)[ 3 ] ; Geng, YJ (Geng, Yijie)[ 3 ] ; Zhang, RL (Zhang, Renli)[ 3 ] ; Ma, HW (Ma, Hanwu)[ 3 ] ; Cheng, JQ (Cheng, Jinquan)[ 3 ] ; Yu, MH (Yu, Muhua)[ 4 ] ; Fang, SS (Fang, Shisong)[ 3 ] 更多内容更少内容 PLOS ONE 卷: 11 期: 5 文献号: e0156017 DOI: 10.1371/journal.pone.0156017 出版年: MAY 25 2016 查看期刊信息 PLOS ONE 出版商 PUBLIC LIBRARY SCIENCE, 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA ISSN: 1932-6203 研究领域 Science & Technology - Other Topics 摘要 A newly emerged H7N9 influenza virus poses high risk to human beings. However, the pathogenic mechanism of the virus remains unclear. The temporal response of primary human alveolar adenocarcinoma epithelial cells (A549) infected with H7N9 influenza virus and H1N1 influenza A virus (H1N1, pdm09) were evaluated using the proteomics approaches (2D-DIGE combined with MALDI-TOF-MS/MS) at 24, 48 and 72 hours post of the infection (hpi). There were 11, 12 and 33 proteins with significant different expressions (P<0.05) at 24, 48 and 72hpi, especially F-actin-capping protein subunit alpha-1 (CAPZA1), Ornithine aminotransferase (OAT), Poly(rC)-binding protein 1 (PCBP1), Eukaryotic translation initiation factor 5A-1 (EIF5A) and Platelet-activating factor acetylhydrolaseIb subunit beta (PAFAH1B2) were validated by western-blot analysis. The functional analysis revealed that the differential proteins in A549 cells involved in regulating cytopathic effect. Among them, the down-regulation of CAPZA1, OAT, PCBP1, EIF5A are related to the death of cells infected by H7N9 influenza virus. This is the first time show that the down-regulation of PAFAH1B2 is related to the later clinical symptoms of patients infected by H7N9 influenza virus. These findings may improve our understanding of pathogenic mechanism of H7N9 influenza virus in proteomics. 关键词 KeyWords Plus  LATELET-ACTIVATING-FACTOR; ORNITHINE-DELTA-AMINOTRANSFERASE; FACTOR ACETYLHYDROLASE; POLY(RC)-BINDING PROTEIN-1; AMINO TRANSFERASE; A(H7N9) VIRUS; H5N1 VIRUS; IDENTIFICATION; MUTATIONS; PCBP1 作者信息 通讯作者地址: Fang, SS (通讯作者) Shenzhen Ctr Dis Control & Prevent, Key Reference Lab Pathogen & Biosafety, Major Infect Dis Control Key Lab, Shenzhen, Peoples R China. 通讯作者地址: Yu, MH (通讯作者) Nanshan Ctr Dis Control & Prevent, Shenzhen, Peoples R China. 地址: [ 1 ] Sun Yat Sen Univ, Sch Publ Hlth, Guangzhou 510275, Guangdong, Peoples R China 增强组织信息的名称 Sun Yat Sen University [ 2 ] Southern Med Univ, Guangzhou, Guangdong, Peoples R China 增强组织信息的名称 Southern Medical University - China [ 3 ] Shenzhen Ctr Dis Control & Prevent, Key Reference Lab Pathogen & Biosafety, Major Infect Dis Control Key Lab, Shenzhen, Peoples R China [ 4 ] Nanshan Ctr Dis Control & Prevent, Shenzhen, Peoples R China 电子邮件地址:1175461964@qq.com; szcdcssf@aliyun.com 基金资助致谢 基金资助机构 授权号 Shenzhen Science and Technology Innovation Project JCYJ20140416095154398 JCYJ20140410164811662 查看基金资助信息关闭基金资助信息 This study was supported by a grant from Shenzhen Science and Technology Innovation Project (JCYJ20140416095154398 & JCYJ20140410164811662) to MHY and SSF. The funders of MHY had a role in study design and the funders of SSF had a role in preparation of the manuscript. 出版商 PUBLIC LIBRARY SCIENCE, 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA 类别 / 分类 研究方向:Science & Technology - Other Topics Web of Science 类别:Multidisciplinary Sciences 文献信息 文献类型:Article 语种:English 入藏号: WOS:000376881700049 PubMed ID: 27223893 ISSN: 1932-6203 其他信息 IDS 号: DN2GI Web of Science 核心合集中的 "引用的参考文献": 50 |
2楼2017-06-14 10:02:55
