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【答案】应助回帖
★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★
感谢参与，应助指数 +1
xiandongzeng: 金币+20, ★★★★★最佳答案 2017-06-14 11:24:33
lazy锦溪: LS-EPI+1 2017-06-14 13:28:18
Preliminary Proteomic Analysis of A549 Cells Infected with Avian Influenza Virus H7N9 and Influenza A Virus H1N1
作者 ing, XM (Ding, Xiaoman)[ 1 ] ; Lu, JH (Lu, Jiahai)[ 1 ] ; Yu, RX (Yu, Ruoxi)[ 2 ] ; Wang, X (Wang, Xin)[ 3 ] ; Wang, T (Wang, Ting)[ 4 ] ; Dong, FY (Dong, Fangyuan)[ 3 ] ; Peng, B (Peng, Bo)[ 3 ] ; Wu, WH (Wu, Weihua)[ 3 ] ; Liu, H (Liu, Hui)[ 3 ] ; Geng, YJ (Geng, Yijie)[ 3 ] ; Zhang, RL (Zhang, Renli)[ 3 ] ; Ma, HW (Ma, Hanwu)[ 3 ] ; Cheng, JQ (Cheng, Jinquan)[ 3 ] ; Yu, MH (Yu, Muhua)[ 4 ] ; Fang, SS (Fang, Shisong)[ 3 ] 更多内容更少内容
PLOS ONE
卷: 11
期: 5
文献号: e0156017
DOI: 10.1371/journal.pone.0156017
出版年: MAY 25 2016
查看期刊信息
PLOS ONE
出版商 PUBLIC LIBRARY SCIENCE, 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA
ISSN: 1932-6203
研究领域 Science & Technology - Other Topics
摘要
A newly emerged H7N9 influenza virus poses high risk to human beings. However, the pathogenic mechanism of the virus remains unclear. The temporal response of primary human alveolar adenocarcinoma epithelial cells (A549) infected with H7N9 influenza virus and H1N1 influenza A virus (H1N1, pdm09) were evaluated using the proteomics approaches (2D-DIGE combined with MALDI-TOF-MS/MS) at 24, 48 and 72 hours post of the infection (hpi). There were 11, 12 and 33 proteins with significant different expressions (P<0.05) at 24, 48 and 72hpi, especially F-actin-capping protein subunit alpha-1 (CAPZA1), Ornithine aminotransferase (OAT), Poly(rC)-binding protein 1 (PCBP1), Eukaryotic translation initiation factor 5A-1 (EIF5A) and Platelet-activating factor acetylhydrolaseIb subunit beta (PAFAH1B2) were validated by western-blot analysis. The functional analysis revealed that the differential proteins in A549 cells involved in regulating cytopathic effect. Among them, the down-regulation of CAPZA1, OAT, PCBP1, EIF5A are related to the death of cells infected by H7N9 influenza virus. This is the first time show that the down-regulation of PAFAH1B2 is related to the later clinical symptoms of patients infected by H7N9 influenza virus. These findings may improve our understanding of pathogenic mechanism of H7N9 influenza virus in proteomics.
关键词
KeyWords Plus LATELET-ACTIVATING-FACTOR; ORNITHINE-DELTA-AMINOTRANSFERASE; FACTOR ACETYLHYDROLASE; POLY(RC)-BINDING PROTEIN-1; AMINO TRANSFERASE; A(H7N9) VIRUS; H5N1 VIRUS; IDENTIFICATION; MUTATIONS; PCBP1
作者信息
通讯作者地址: Fang, SS (通讯作者) Shenzhen Ctr Dis Control & Prevent, Key Reference Lab Pathogen & Biosafety, Major Infect Dis Control Key Lab, Shenzhen, Peoples R China.
通讯作者地址: Yu, MH (通讯作者) Nanshan Ctr Dis Control & Prevent, Shenzhen, Peoples R China.
地址: [ 1 ] Sun Yat Sen Univ, Sch Publ Hlth, Guangzhou 510275, Guangdong, Peoples R China
增强组织信息的名称
Sun Yat Sen University
[ 2 ] Southern Med Univ, Guangzhou, Guangdong, Peoples R China
增强组织信息的名称
Southern Medical University - China
[ 3 ] Shenzhen Ctr Dis Control & Prevent, Key Reference Lab Pathogen & Biosafety, Major Infect Dis Control Key Lab, Shenzhen, Peoples R China
[ 4 ] Nanshan Ctr Dis Control & Prevent, Shenzhen, Peoples R China
电子邮件地址:1175461964@qq.com; szcdcssf@aliyun.com
基金资助致谢
基金资助机构 授权号
Shenzhen Science and Technology Innovation Project JCYJ20140416095154398
JCYJ20140410164811662
查看基金资助信息关闭基金资助信息
This study was supported by a grant from Shenzhen Science and Technology Innovation Project (JCYJ20140416095154398 & JCYJ20140410164811662) to MHY and SSF. The funders of MHY had a role in study design and the funders of SSF had a role in preparation of the manuscript.
出版商
PUBLIC LIBRARY SCIENCE, 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA
类别 / 分类
研究方向:Science & Technology - Other Topics
Web of Science 类别:Multidisciplinary Sciences
文献信息
文献类型:Article
语种:English
入藏号: WOS:000376881700049
PubMed ID: 27223893
ISSN: 1932-6203
其他信息
IDS 号: DN2GI
Web of Science 核心合集中的 "引用的参考文献": 50 |
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