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[求助]
透明质酸醛基化 已有2人参与
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透明质酸用高碘酸钠氧化出醛基 测定醛基含量 a volume of 25 μl (0.6%) oxidized HA and 25 μl (30 mM) t-BC in 1% aqueous trichloroacetic acid were well mixed and allowed to react in a disposable Eppendorf tube at room temperature. After 24 h, 0.5 ml of aqueous TNBS solution(6 mM, 0.1 M borate buffer, pH 8) was transferred into the eppendorf tube to react with the excess t-BC. The t-BC-TNBS reaction was allowed to react for 60 min at room temperature. 这段话中是用三氯乙酸的量没有说明，是多少无所谓的意思吗？ 还有TNBS solution(6 mM, 0.1 M borate buffer, pH 8)是什么意思？ 求指教！！ |
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