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【答案】应助回帖
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感谢参与，应助指数 +1
dengminghua: 金币+15 2017-03-13 22:08:26
lazy锦溪: LS-EPI+1 2017-03-13 22:58:15
Cloning, sequence characterization and expression analyses of PK gene from pepper cytoplasmic male sterility (CMS)
作者:Lv, JH (Lv, Jun-heng)[ 1,2 ] ; Huo, JL (Huo, Jin-long)[ 1 ] ; Zhao, K (Zhao, Kai)[ 1 ] ; Deng, MH (Deng, Ming-hua)[ 1 ]
RESEARCH JOURNAL OF BIOTECHNOLOGY
卷: 10 期: 12 页: 40-46
出版年: DEC 2015
查看期刊信息
摘要
Pyruvate kinase (PK) is an important regulatory enzyme of the pathways of both anaerobic glycolysis and gluconeogenesis, it plays an important function in energy metabolism. The complete coding sequence (CDS) of the PK gene was amplified using a reverse transcriptase PCR based on the conserved sequence information of the tomato and other Solanaceae plants and known highly homologous pepper ESTs. 510 amino acids were encoded by the 1533 long cDNA of the pepper PK gene. Nucleotide sequence analysis showed that the encoded amino acids were highly homologous with the seven species: Solanum lycopersicum (97%), Solanum tuberosum (95%), Jatropha curcas (93%), Phaseolus vulgaris (93%), Vitis vinifera (92%), Prunus persica (92%) and Glycine max (92%).
The result of the tissue expression showed that the pepper PK gene is over expressed in placenta, seed and flower, moderately in pericarp and stem, weakly in leaf. During the abortion stages, expression levels of PK in anthers of a sterile line were higher than that in the maintainer line.
关键词
作者关键词:Capsicum annuum L.; Pyruvate kinase; Cloning; Sequence characterization; Cytoplasmic male sterility; Energy metabolism
KeyWords Plus:BRASSICA-NAPUS; POLLEN DEVELOPMENT; PYRUVATE-KINASE; HIGHER-PLANTS; PREDICTION; FERTILITY; LINES; RICE
作者信息
通讯作者地址: Lv, JH (通讯作者)
显示增强组织信息的名称 Yunnan Agr Univ, Kunming 650201, Peoples R China.
地址:
显示增强组织信息的名称 [ 1 ] Yunnan Agr Univ, Kunming 650201, Peoples R China
显示增强组织信息的名称 [ 2 ] Hunan Univ, Changsha 410082, Hunan, Peoples R China
电子邮件地址:pepper_breed@sina.com
基金资助致谢
基金资助机构 授权号
Foundation of Natural Science of Yunnan Province, China
2013FB038
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出版商
RESEARCH JOURNAL BIOTECHNOLOGY, SECTOR A-80, SCHEME NO 54, VIJAY NAGAR, A B ROAD, INDORE, 452 010 MP, INDIA
类别 / 分类
研究方向:Biotechnology & Applied Microbiology
Web of Science 类别:Biotechnology & Applied Microbiology
文献信息
文献类型:Article
语种:English
入藏号: WOS:000365769300007
ISSN: 2278-4535
其他信息
IDS 号: CX5UZ
Web of Science 核心合集中的 "引用的参考文献": 27
Web of Science 核心合集中的 "被引频次": 0 |
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