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dengminghua木虫 (著名写手)
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Cloning, sequence characterization and expression analyses of PK gene from pepper cytoplasmic male sterility (CMS). Res J Biotech, 10(12): 40-46, 2015. Molecular cloning sequence characterization of a novel pepper gene CaNDPK and its effect on cytoplasmic male sterility. Res J Biotech, 11(4): 36-41, 2016. cDNA cloning and molecular characteristics of two pepper genes H2A and H4. J Anim Plant Sci, 2015, 25(1): 278-283 麻烦帮忙检索以上三篇文章，告知检索号，谢谢 |
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lzg020716
木虫 (文学泰斗)
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【答案】应助回帖
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感谢参与，应助指数 +1
dengminghua: 金币+15 2017-03-13 22:08:26
lazy锦溪: LS-EPI+1 2017-03-13 22:58:15
感谢参与，应助指数 +1
dengminghua: 金币+15 2017-03-13 22:08:26
lazy锦溪: LS-EPI+1 2017-03-13 22:58:15
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Cloning, sequence characterization and expression analyses of PK gene from pepper cytoplasmic male sterility (CMS) 作者:Lv, JH (Lv, Jun-heng)[ 1,2 ] ; Huo, JL (Huo, Jin-long)[ 1 ] ; Zhao, K (Zhao, Kai)[ 1 ] ; Deng, MH (Deng, Ming-hua)[ 1 ] RESEARCH JOURNAL OF BIOTECHNOLOGY 卷: 10 期: 12 页: 40-46 出版年: DEC 2015 查看期刊信息 摘要 Pyruvate kinase (PK) is an important regulatory enzyme of the pathways of both anaerobic glycolysis and gluconeogenesis, it plays an important function in energy metabolism. The complete coding sequence (CDS) of the PK gene was amplified using a reverse transcriptase PCR based on the conserved sequence information of the tomato and other Solanaceae plants and known highly homologous pepper ESTs. 510 amino acids were encoded by the 1533 long cDNA of the pepper PK gene. Nucleotide sequence analysis showed that the encoded amino acids were highly homologous with the seven species: Solanum lycopersicum (97%), Solanum tuberosum (95%), Jatropha curcas (93%), Phaseolus vulgaris (93%), Vitis vinifera (92%), Prunus persica (92%) and Glycine max (92%). The result of the tissue expression showed that the pepper PK gene is over expressed in placenta, seed and flower, moderately in pericarp and stem, weakly in leaf. During the abortion stages, expression levels of PK in anthers of a sterile line were higher than that in the maintainer line. 关键词 作者关键词:Capsicum annuum L.; Pyruvate kinase; Cloning; Sequence characterization; Cytoplasmic male sterility; Energy metabolism KeyWords Plus:BRASSICA-NAPUS; POLLEN DEVELOPMENT; PYRUVATE-KINASE; HIGHER-PLANTS; PREDICTION; FERTILITY; LINES; RICE 作者信息 通讯作者地址: Lv, JH (通讯作者) 显示增强组织信息的名称 Yunnan Agr Univ, Kunming 650201, Peoples R China. 地址: 显示增强组织信息的名称 [ 1 ] Yunnan Agr Univ, Kunming 650201, Peoples R China 显示增强组织信息的名称 [ 2 ] Hunan Univ, Changsha 410082, Hunan, Peoples R China 电子邮件地址:pepper_breed@sina.com 基金资助致谢 基金资助机构 授权号 Foundation of Natural Science of Yunnan Province, China 2013FB038 查看基金资助信息 出版商 RESEARCH JOURNAL BIOTECHNOLOGY, SECTOR A-80, SCHEME NO 54, VIJAY NAGAR, A B ROAD, INDORE, 452 010 MP, INDIA 类别 / 分类 研究方向:Biotechnology & Applied Microbiology Web of Science 类别:Biotechnology & Applied Microbiology 文献信息 文献类型:Article 语种:English 入藏号: WOS:000365769300007 ISSN: 2278-4535 其他信息 IDS 号: CX5UZ Web of Science 核心合集中的 "引用的参考文献": 27 Web of Science 核心合集中的 "被引频次": 0 |
2楼2017-03-13 21:24:11
lzg020716
木虫 (文学泰斗)
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【答案】应助回帖
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dengminghua: 金币+15 2017-03-13 22:08:34
dengminghua: 金币+15 2017-03-13 22:08:34
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Molecular cloning, sequence characterization of a novel pepper gene CaNDPK and its effect on cytoplasmic male sterility 作者:Lv, JH (Lv Jun-Heng)[ 1,2 ] ; Zhao, K (Zhao Kai)[ 1 ] ; Huo, JL (Huo Jin-Long)[ 1 ] ; Deng, MH (Deng Ming-Hua)[ 1 ] RESEARCH JOURNAL OF BIOTECHNOLOGY 卷: 11 期: 4 页: 36-41 出版年: APR 2016 查看期刊信息 摘要 Nucleoside diphosphate kinase (NDPK) is a key enzyme which catalyzes the transfer of the gamma phosphates from NTP to NDP. NDPK is considered as housekeeping enzyme involved in energy metabolism and homeostasis of intracellular NTP pools. The complete coding sequence (CDS) of the CaNDPK gene was amplified using a reverse transcriptase PCR based on the conserved sequence information of the tomato and other Solanaceae plants and known highly homologous pepper ESTs. 148 amino acids were encoded by the 447 long cDNA of the pepper CaNDPK gene. Nucleotide sequence analysis showed that the encoded amino acids were highly homologous with the seven species, Nicotiana tomentosiformis (97%), Nicotiana sylvestris (97%), Solanum lycopersicum (95%), Coffea canephora (94%), Flaveria bidentis (93%), Jatropha curcas (92%) and Camellia sinensis (90%). The result of the tissues expression showed that the pepper CaNDPK gene is over expressed in placenta, moderately in seed, weakly in stem, leaf, flower and pericarp. During the abortion stages, expression levels of CaNDPK in anthers of the sterile line were different with that in the maintainer and the CaNDPK gene expression level of transcripts in the maintainer line is much more stable than that in the sterile line. The unusual and the instable expression levels of CaNDPK may disturb the balance of energy metabolism in the sterile line indicating that stable transcripts of CaNDPK are necessary to maintain energy metabolism at a normal level. The CaNDPK gene plays an important role in keeping the balance of the energy metabolism within normal levels during microspore development. 关键词 作者关键词:Capsicum annuum L.; Nucleoside diphosphate kinase; Cloning; Sequence characterization; Cytoplasmic male sterility KeyWords Plus:NUCLEOSIDE-DIPHOSPHATE KINASE; ELECTRON-TRANSPORT; POLLEN DEVELOPMENT; OXIDATIVE STRESS; MITOCHONDRIA; PREDICTION; SIGNALS 作者信息 通讯作者地址: Deng, MH (通讯作者) 显示增强组织信息的名称 Yunnan Agr Univ, Kunming 650201, Peoples R China. 地址: 显示增强组织信息的名称 [ 1 ] Yunnan Agr Univ, Kunming 650201, Peoples R China 显示增强组织信息的名称 [ 2 ] Hunan Univ, Changsha 410082, Hunan, Peoples R China 电子邮件地址:pepper_breed@sina.com 基金资助致谢 基金资助机构 授权号 Foundation of Natural Science of Yunnan Province, China 2013FB038 查看基金资助信息 出版商 RESEARCH JOURNAL BIOTECHNOLOGY, SECTOR A-80, SCHEME NO 54, VIJAY NAGAR, A B ROAD, INDORE, 452 010 MP, INDIA 类别 / 分类 研究方向:Biotechnology & Applied Microbiology Web of Science 类别:Biotechnology & Applied Microbiology 文献信息 文献类型:Article 语种:English 入藏号: WOS:000374989500005 ISSN: 2278-4535 其他信息 IDS 号: DK5VS Web of Science 核心合集中的 "引用的参考文献": 30 Web of Science 核心合集中的 "被引频次": 0 |
3楼2017-03-13 21:25:30
lzg020716
木虫 (文学泰斗)
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4楼2017-03-13 21:29:07
dengminghua
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5楼2017-03-13 22:08:09