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Biomaterials关于介孔硅生物相容性的最新文章
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很不错，推荐 2009年发表的文章，题目是2009The compatibility of hepatocytes with chemically modified porous silicon with reference to in vitro biosensors abstract如下： PorousSiisananostructuredmaterialthatisofinterestformolecularandcell-basedbiosensing,drug livery,andtissueengineeringapplications.Surfacechemistryisanimportantfactordeterminingthe stabilityofporousSiinaqueousmedia,itsafnityforvariousbiomolecularspecies,anditscompatibility withtissues.Inthisstudy,theattachmentandviabilityofaprimarycelltypetoporousSisamples containingvarioussurfacechemistriesisreported,andtheabilityoftheporousSilmstoretaintheir opticalreectivitypropertiesrelevanttomolecularbiosensingisassessed.Fourchemicalspeciesgrafted totheporousSisurfacearestudied:siliconoxide(viaozoneoxidation),dodecyl(viahydrosilylationwith dodecene),undecanoicacid(viahydrosilylationwithundecylenicacid),andoligo(ethylene)glycol(via hydrosilylationwithundecylenicacidfollowedbyanoligo(ethylene)glycolcouplingreaction).Fourier TransformInfrared(FTIR)spectroscopyandcontactanglemeasurementsareusedtocharacterizethe surface.Adhesionandshort-termviabilityofprimaryrathepatocytesonthesesurfaces,withand withoutpre-adsorptionofcollagentypeI,areassessedusingvitaldyes(calcein-AMandethidium homodimerI).Cellviabilityonundecanoicacid-terminatedporousSi,oxide-terminatedporousSi,and oxide-terminatedat(non-porous)Siaremonitoredbyquanticationofalbuminproductionoverthe courseof8days.ThestabilityofporousSithinlmsafter8daysincellcultureisprobedbymeasuring theopticalinterferometricreectancespectra.Resultsshowthathepatocytesadherebettertosurfaces coatedwithcollagen,andthatchemicalmodicationdoesnotexertadeleteriouseffectonprimaryrat hepatocytes.ThehydrosilylationchemistrygreatlyimprovesthestabilityofporousSiincontactwith culturedprimarycellswhileallowingcellcoveragelevelscomparabletostandardculturepreparations ontissueculturepolystyrene http://www.namipan.com/d/349befe80c8fd3c3224d3eb811589a9ae5157dc6f01b1d00 [ Last edited by ltlipc on 2008-12-19 at 21:01 ] |
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