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yuguiyan

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[求助] 帮我看看检索了没有

14-3-3γ affects eIF5 to regulate β-casein synthesis in bovine mammary epithelial cells
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1楼2017-02-09 16:50:48
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yuguiyan: 金币+5, ★★★★★最佳答案 2017-02-13 15:14:14
lazy锦溪: LS-EPI+1 2017-02-13 15:49:52
14-3-3 gamma affects eIF5 to regulate beta-casein synthesis in bovine mammary epithelial cells
作者:Yu, CP (Yu, Cuiping)[ 1,2 ] ; Luo, CC (Luo, Chaochao)[ 2 ] ; Gu, XY (Gu, Xinyu)[ 2 ] ; Zang, YL (Zang, Yanli)[ 2 ] ; Qu, B (Qu, Bo)[ 2 ] ; Khudhair, N (Khudhair, Nagam)[ 2 ] ; Li, QZ (Li, Qingzhang)[ 2 ] ; Gao, XJ (Gao, Xuejun)[ 2 ]
CANADIAN JOURNAL OF ANIMAL SCIENCE
卷: 96  期: 4  页: 478-487
DOI: 10.1139/cjas-2016-0038
出版年: DEC 2016
查看期刊信息
摘要
The 14-3-3 gamma protein participates in many biological processes; however, its regulatory mechanism in milk protein synthesis is not well studied. We hypothesized that 14-3-3 gamma might affect eIF5 (an initiation factor) to regulate beta-casein synthesis in dairy cows. In this study, a possible interaction between 14-3-3 gamma and eIF5 was investigated using bovine mammary epithelial cells (BMECs). The expression levels of 14-3-3 gamma and eIF5 in the mammary gland tissues from cows producing higher quality milk were higher than those from cows producing low-quality milk. Moreover, the expression of 14-3-3 gamma, eIF5, and beta-casein were increased at both mRNA and protein levels in BMECs cultured in vitro with methionine (Met) supplementation. Coimmunoprecipitation, colocalization, and FRET analysis further showed the evidences that 14-3-3 gamma physically bound to eIF5 in BMECs. Gene function studies revealed that 14-3-3 gamma positively regulated eIF5 through alteration of eIF2 alpha/p-eIF2 alpha ratio. Collectively, our data suggest that 14-3-3 gamma regulates beta-casein translation in BMECs through interaction with eIF5.
关键词
作者关键词:14-3-3 gamma; eIF5; eIF2 alpha; methionine; beta-casein synthesis; bovine mammary epithelial cells
KeyWords Plus:MILK PROTEIN-SYNTHESIS; DAIRY-COW; EUKARYOTIC TRANSLATION; PREINITIATION COMPLEX; SIGNALING PATHWAY; PROLIFERATION; PHOSPHORYLATION; EXPRESSION; LACTATION; PROMOTES
作者信息
通讯作者地址: Gao, XJ (通讯作者)
显示增强组织信息的名称        Northeast Agr Univ, Key Lab Agr Biol Funct Genes, Harbin 150030, Peoples R China.
地址:
显示增强组织信息的名称        [ 1 ] Dalian Polytech Univ, Natl Engn Res Ctr Seafood, Sch Food Sci & Technol, Dalian 116034, Peoples R China
显示增强组织信息的名称        [ 2 ] Northeast Agr Univ, Key Lab Agr Biol Funct Genes, Harbin 150030, Peoples R China
电子邮件地址:gaoxj5390@sina.com
基金资助致谢
基金资助机构        授权号
863 Project of Ministry of Science and Technology of China        
2013AA102504-03
Major State Basic Research Development Program of China (973 Program)        
2011CB100804
Doctoral Program of Dalian Polytechnic University        
61020705
查看基金资助信息   
出版商
CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS, 65 AURIGA DR, SUITE 203, OTTAWA, ON K2E 7W6, CANADA
类别 / 分类
研究方向:Agriculture
Web of Science 类别:Agriculture, Dairy & Animal Science
文献信息
文献类型:Article
语种:English
入藏号: WOS:000388368200005
ISSN: 0008-3984
eISSN: 1918-1825
其他信息
IDS 号: EC8CP
Web of Science 核心合集中的 "引用的参考文献": 31
Web of Science 核心合集中的 "被引频次": 0
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https://apps.webofknowledge.com/ ... age=1&doc=1
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yuguiyan

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引用回帖:
2楼: Originally posted by baiyuefei at 2017-02-09 18:17:56
14-3-3 gamma affects eIF5 to regulate beta-casein synthesis in bovine mammary epithelial cells
作者:Yu, CP (Yu, Cuiping) ; Luo, CC (Luo, Chaochao) ; Gu, XY (Gu, Xinyu) ; Zang, YL (Zang, Yanli) ; Qu, ...

这是已经检索了是吧？
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4楼2017-02-13 15:14:52
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baiyuefei

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引用回帖:
4楼: Originally posted by yuguiyan at 2017-02-13 15:14:52
这是已经检索了是吧？...

嗯
5楼2017-02-13 15:16:21
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yuguiyan

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引用回帖:
5楼: Originally posted by baiyuefei at 2017-02-13 15:16:21
嗯

多谢
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