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| 14-3-3γ affects eIF5 to regulate β-casein synthesis in bovine mammary epithelial cells |
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baiyuefei
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yuguiyan: 金币+5, ★★★★★最佳答案 2017-02-13 15:14:14
lazy锦溪: LS-EPI+1 2017-02-13 15:49:52
感谢参与，应助指数 +1
yuguiyan: 金币+5, ★★★★★最佳答案 2017-02-13 15:14:14
lazy锦溪: LS-EPI+1 2017-02-13 15:49:52
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14-3-3 gamma affects eIF5 to regulate beta-casein synthesis in bovine mammary epithelial cells 作者:Yu, CP (Yu, Cuiping)[ 1,2 ] ; Luo, CC (Luo, Chaochao)[ 2 ] ; Gu, XY (Gu, Xinyu)[ 2 ] ; Zang, YL (Zang, Yanli)[ 2 ] ; Qu, B (Qu, Bo)[ 2 ] ; Khudhair, N (Khudhair, Nagam)[ 2 ] ; Li, QZ (Li, Qingzhang)[ 2 ] ; Gao, XJ (Gao, Xuejun)[ 2 ] CANADIAN JOURNAL OF ANIMAL SCIENCE 卷: 96 期: 4 页: 478-487 DOI: 10.1139/cjas-2016-0038 出版年: DEC 2016 查看期刊信息 摘要 The 14-3-3 gamma protein participates in many biological processes; however, its regulatory mechanism in milk protein synthesis is not well studied. We hypothesized that 14-3-3 gamma might affect eIF5 (an initiation factor) to regulate beta-casein synthesis in dairy cows. In this study, a possible interaction between 14-3-3 gamma and eIF5 was investigated using bovine mammary epithelial cells (BMECs). The expression levels of 14-3-3 gamma and eIF5 in the mammary gland tissues from cows producing higher quality milk were higher than those from cows producing low-quality milk. Moreover, the expression of 14-3-3 gamma, eIF5, and beta-casein were increased at both mRNA and protein levels in BMECs cultured in vitro with methionine (Met) supplementation. Coimmunoprecipitation, colocalization, and FRET analysis further showed the evidences that 14-3-3 gamma physically bound to eIF5 in BMECs. Gene function studies revealed that 14-3-3 gamma positively regulated eIF5 through alteration of eIF2 alpha/p-eIF2 alpha ratio. Collectively, our data suggest that 14-3-3 gamma regulates beta-casein translation in BMECs through interaction with eIF5. 关键词 作者关键词:14-3-3 gamma; eIF5; eIF2 alpha; methionine; beta-casein synthesis; bovine mammary epithelial cells KeyWords Plus:MILK PROTEIN-SYNTHESIS; DAIRY-COW; EUKARYOTIC TRANSLATION; PREINITIATION COMPLEX; SIGNALING PATHWAY; PROLIFERATION; PHOSPHORYLATION; EXPRESSION; LACTATION; PROMOTES 作者信息 通讯作者地址: Gao, XJ (通讯作者) 显示增强组织信息的名称 Northeast Agr Univ, Key Lab Agr Biol Funct Genes, Harbin 150030, Peoples R China. 地址: 显示增强组织信息的名称 [ 1 ] Dalian Polytech Univ, Natl Engn Res Ctr Seafood, Sch Food Sci & Technol, Dalian 116034, Peoples R China 显示增强组织信息的名称 [ 2 ] Northeast Agr Univ, Key Lab Agr Biol Funct Genes, Harbin 150030, Peoples R China 电子邮件地址:gaoxj5390@sina.com 基金资助致谢 基金资助机构 授权号 863 Project of Ministry of Science and Technology of China 2013AA102504-03 Major State Basic Research Development Program of China (973 Program) 2011CB100804 Doctoral Program of Dalian Polytechnic University 61020705 查看基金资助信息 出版商 CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS, 65 AURIGA DR, SUITE 203, OTTAWA, ON K2E 7W6, CANADA 类别 / 分类 研究方向:Agriculture Web of Science 类别:Agriculture, Dairy & Animal Science 文献信息 文献类型:Article 语种:English 入藏号: WOS:000388368200005 ISSN: 0008-3984 eISSN: 1918-1825 其他信息 IDS 号: EC8CP Web of Science 核心合集中的 "引用的参考文献": 31 Web of Science 核心合集中的 "被引频次": 0 |
2楼2017-02-09 18:17:56
baiyuefei
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3楼2017-02-09 18:18:07
yuguiyan
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4楼2017-02-13 15:14:52
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5楼2017-02-13 15:16:21
yuguiyan
至尊木虫 (文坛精英)
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6楼2017-02-17 17:02:44
