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zixiao_226

铜虫 (小有名气)

应助: 8 (幼儿园)
金币: 314.3
帖子: 68
在线: 14.5小时
虫号: 496543
注册: 2008-01-19
专业: 生物化学

[求助] 如何回复Genbank序列里含有读码框移位问题和含有终止密码子问题

早前给Genbank上传了序列，已经给登录号了，最近收到回信说校准过程中发现读码框移位问题，让给出解释。序列是自己pcr获得的一段蛋白因子的，含有内含子，测序回来后直接blast建树。这种情况如何回复，序列需要怎么校准，还是编码框起始位置需要修改?回信的校准结果怎么看？请各位大师帮助！以下是信得内容，以及部分序列。
Upon doing an alignment of your sequences, there may be reading
frame shifts in many of the sequences near the 5' and 3' ends.

If the nucleotide sequences are incorrect, send us the corrected
sequences, and we will replace the current sequences in your
submission.

If the sequences are correct, please let us know if you have any
evidence that these frame shifted proteins are translated.

alignment results:

1 50
KX094899 ...CCAATGTCA.TCGTATC.GGCC.CGTCGACTCTGGCAAGTCGACCAC
KX094919 ...CACAAGT.G.TCGTATC.GGCC.CGTCGACTCTGGCAAGTCGACCAC
KX094908 ....CAGAGTCA.TGGTATC.GGCCACGTCGACTCTGGCA.GTCGACCAC
KX094918 ....CCAAAAAG.TCGTATC.GGTC.CGTCGACTCTGGCAAGTCGACCAC
KX094916 ...CCAG...CG.TCGTATC.GGCA.CGTCGACTCTGGCA.GTCGACCAC
KX094910 .....CATCGTG.TCGTATC.GGCCACGTCGACTCTGGCAAGTCGACCAC
KX094901 ...CCAATGTG..TCGT.ATCGGC.ACGTCGACTCTGGCAAGTCGACCAC
KX094905 ...CCAATGTTGATCGTCATCGGCCACGTCGACTCTGGCAAGTCGACCAC
KX094909 ..ACCCTTTTGGTTCGTCATCGGCCACGTCGACTCTGGCA.GTCGACCAC
KX094915 ....ACTTGTGG.TCGTCATCGGCCACGTCGACTCTGGCA.GTCGACCAC
KX094902 ..CAAATTGCCA.ACGCCTCGGGCC.CGTCGACTCTGGCAAGTCGACCAC
KX094920 ...CCAATGACG.TCGTATCGG.CC.CGTCGACTCTGGCAAGTCGACCAC
KX094926 ..ACCCTTATCA.TTGTCTCGG.CC.CGTCGACTCTGGCA.GTCGACCAC
KX094921 ...CACCTATCA.T.GTATCGG.CA.CGTCGACTCTGGCAAGTCGACCAC
KX094904 ..TACAAACTCG.TCGTATCGGGCCACGTCGACTCTGGCAAGTCGACCAC
KX094907 ....CCCTGACG.ATGTCTCGGCC..CGTCGACTCTGGCA.GTCGACCAC
KX094923 ....CCCCAGCG.TCGTATCGGCC...GTCGACTCTGGCA.GTCGACCAC
KX094903 ..CATTGAACCAATGGTCATCGGCCACGTCGACTCTGGCA.GTCGACCAC
KX094917 ..CCT...CACGTCGTGTATCGGC.ACGTCGACTCTGGCA.GTCGACCAC
KX094924 ........TCTCACTCGTCTCGGC.ACGTCGACTCTGGCA.GTCGACCAC
KX094900 .....CCATTCAT.CGT.ATCGGCCACGTCGACTCTGGCAAGTCGACCAC
KX094911 ...CCACTTTCGTTCGTCATCGGCCACGTCGACTCTGGCAAGTCGACCAC
KX094913 ...CCACTTTCGT.CGT.ATCGGC.ACGTCGACTCTGGCAAGTCGACCAC
KX094906 .....CCCGTCGATCGT.CATCGGCACGTCGACTCTGGCAAGTCGACCAC
KX094912 AAAAATTTGTCGTTTAT.CGGGCCACGATCGACTCTGGCAAGTCGACCAC
KX094914 ...GGATCATCGGTTGT.CGCTATCAAGTCGACTCTGGCAGTCAGACCAC
KX094925 ...CCACCTTCG.TCGT.CTCGGCC..GTCGACTCTGGCA.GTCGACCAC
KX094922 ....CCATGTCCATCGTCATCGGCC.CGTCGAC

251 300
KX094899 CCTTT.GCCCATCGATTTCCCCTACGA.CTCG..................
KX094919 CCTTT.GCCCATCGATTTCCCCTACGA.CTCG..................
KX094908 CCTTT.GCCCATCGACTTCCCCTACGA.CTCG..................
KX094918 CCTTT.GCCCATCGACTTCCCCTACGA.CTCG..................
KX094916 CCTTT.GCCCATCGATTTCCCCTACGA.CTCG..................
KX094910 CCTTTTGCCCATCGATTTTCCCTTCGA.CTCGCCGCTCCCATACAACTCG
KX094901 GCT....CCCATCGATT...CCCACGA.TTCGCTCCCTC......ACTCG
KX094905 GCT....CCCATCGATT...CCCACGA.TTCGCTCCCTC......ACTCG
KX094909 GCT....CCCATCGATT...CCCACGA.TTCGCTCCCTC......ACTCG
KX094915 GCT....CCCATCGATT...CCCACGA.TTCGCTCCCTC......ACTCG
KX094902 GCT....CCCATCGATT...CCCACGA.TTCGCTCCCTC......ACTCG
KX094920 GCT....CCCATCGATT...CCCACGA.TTCGCTCCCTC......ACTCG
KX094926 GCT....CCCATCGATT...CCCACGA.TTCGCTCCCTC......ACTCG
KX094921 GCT....CCCATCGATT...CCCACGA.TTCGCTCCCTC......ACTCG
KX094904 GCT....CCCATCGATT...CCCACGA.TTCGCTCCCTC......ACTCG
KX094907 GCT....CCCATCGATT...CCCACGA.TTCGCTCCCTC......ACTCG
KX094923 GCT....CCCATCGATT...CCCACGA.TTCGCTCCCTC......ACTCG
KX094903 GCT....CCCATCGATT...CCCACGA.TTCGCTCCCTC......ACTCG
KX094917 GCT....CCCATCGATT...CCCACGA.TTCGCTCCCTC......ACTCG
KX094924 GCT....CCCATCGATT...CCCACGA.TTCGCTCCCTC......ACTCG
KX094900 GCTATCCCACATCGAATTCCCCGTCGAATTCCCTCCTCC...GCGACACG
KX094911 GCTATCCCACATCGAATTCCCCGTCGAATTCCCTCCTCC...GCGACACG
KX094913 GCTATCCCACATCGAATTCCCCGTCGAATTCCCTCCTCC...GCGACACG
KX094906 GCTATTCCACATCGAATTCCCCGTCGAATTCCCTCCTCC...GCGACACG
KX094912 GCTATTCCACATCGAATTCCCCGTCGAATTCCCTCCTCC...GCGACACG
KX094914 GCTATTCCACATCGAATTCCCCGTCGAATTCCCTCCTCC...GCGACACG
KX094925 GCTATTCCACATCGAATTCCCCGTCGAATTCCCTCCTCC...GCGACACG
KX094922 GATATTCCACATCGAATTCCCCGTCGAATTCCCTCCATC...GCGATACG

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